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Method for improving test-tube baby clinical outcome by selecting embryo with good development potential

A technology of developing potential and embryos, which is applied in the field of reproductive medicine science to achieve the effect of increasing the implantation rate of embryos, improving pregnancy outcomes, and increasing the number of live births of babies

Inactive Publication Date: 2016-10-12
黄官友
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, we used liquid chip high-throughput protein analysis technology (LHTPA) to detect the contents of TNF-α, IL-6, IL-1β and GM-CSF in the culture medium of human pre-implantation embryos. Cytokines can predict embryonic developmental potential and clinical outcome, so this data is unpublished

Method used

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  • Method for improving test-tube baby clinical outcome by selecting embryo with good development potential
  • Method for improving test-tube baby clinical outcome by selecting embryo with good development potential
  • Method for improving test-tube baby clinical outcome by selecting embryo with good development potential

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Embodiment approach

[0018] Implementation method, this method comprises the following steps:

[0019](1) On the day of transplantation, the culture medium for culturing the embryos to be transplanted before each embryo transfer of each in vitro fertilization recipient is collected. On the first day after IVF, after fertilization was confirmed, each fertilized egg of each IVF recipient was placed in 30-50 μL of 10% human serum albumin (SAGE In-Vitro Fertilization, Inc.; Trumbull, CT, USA) in the cleavage fluid (ART-1026, SAGE In-Vitro Fertilization, Inc.; Trumbull, CT, USA) droplet, set 37 ℃, 5% CO 2 Continue to culture in the incubator for 2 days until the embryo transfer; now, the embryos have been cultured in vitro to the 3rd day, 4h-6h before the embryo transfer, according to the morphological evaluation based on cleavage rate and morphology Evaluate the quality of the embryos to be transplanted for each IVF recipient, and each IVF recipient transfers 2-3 embryos at a time; after the embryos ...

Embodiment 1

[0027] 2714 (Medical record number) Infertile couple, woman's name, Chen X, 33 years old, man's name, Qin XX, diagnosis: secondary infertility: left tubal obstruction after Lap surgery; intrauterine adhesions after hysteroscopy; woman in 2014 Egg retrieval was performed on January 17, and the embryo transfer operation was performed on January 20, 2014; the concentration of IL8 in the culture medium of the transferred embryo was determined to be 0.21pg / ml before the embryo transfer operation, and the grade of the transferred embryo was 2 / 5 and 2 / 7; 14 days after the embryo transfer operation, the woman's blood test was positive for hCG. On September 25, 2014, a live baby boy was delivered by cesarean section, weighing 3.2 kg.

Embodiment 2

[0029] 5124 (Medical record number) The name of the infertile woman, Yang XX, 30 years old, the name of the man, party X, diagnosis: primary infertility: after pelvic severe adhesion separation; pelvic tuberculosis (old); the woman died on January 1, 2014 The eggs were retrieved on January 18, and the embryo transfer operation was performed on January 21, 2014; the concentration of IL8 in the culture medium of the transferred embryos was determined to be 0.27pg / ml before the embryo transfer operation, and the grades of the transferred embryos were 1 / 5 and 1 / 7; 14 days after the embryo transfer operation, the woman's blood test was positive for hCG. On October 5, 2014, a live baby boy was delivered by cesarean section, weighing 3.1 kg.

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Abstract

The invention discloses a method for improving the test-tube baby clinical outcome by selecting an embryo with good development potential. The method comprises five steps that the transplanting embryo with good development potential is selected mainly according to IL-8 in an embryo culture solution before the transplanting; concretely, a liquid phase chip high-flux protein analysis technology is used for detecting the content of IL-8 in a sample; an IL-8 positive embryo in the culture solution is selected to be combined with a morphology evaluation method to perform transplantation; the IL-8 positive embryo in the culture solution before the transplantation is used for being combined with the morphological high-quality embryo to perform transplantation; the pregnancy rate can be improved by more than 8 percent, and can reach 60.76 percent; compared with the existing transplantation method for selecting the embryo singly by a morphology method, the method provided by the invention has the advantages that the pregnancy rate can be improved by more than 8 percent to 12 percent. When the method is combined with the existing morphology method for selecting high-quality embryo; or the high-quality embryo and the ordinary embryo are transplanted to an in vitro fertilization person, the embryo planting rate can also be improved; the baby live birth number is increased; the patient clinic pregnancy rate is improved; the abortion rate is reduced.

Description

technical field [0001] The invention belongs to the field of reproductive medicine science and test-tube baby transplantation, and specifically relates to the selection of interleukin-8 (IL-8) by measuring the content of interleukin-8 (IL-8) in the culture medium for culturing embryos to be transplanted before human embryo transplantation before test-tube baby transplantation. Embryos of good developmental potential are transferred to improve pregnancy outcomes. Background technique [0002] As the use of assisted reproductive technology, or IVF, increases worldwide, the rapid and non-invasive selection of the best embryos for transfer remains an important issue in reproductive medicine. For most IVF patients, in order to obtain higher implantation rates (IRs) and pregnancy rates, multiple embryos are transferred per transfer, which can lead to multiple pregnancies. The increase in multiple pregnancies due to in vitro fertilization (IVF / IVSI) has exposed society to a series...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6869
Inventor 黄官友
Owner 黄官友
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