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Method for quantification of peri-implantation window phase endometrial pinopodes

A technology of endometrium and pinocytosis, applied in measuring devices, material analysis using wave/particle radiation, instruments, etc., can solve problems such as poor accuracy and subjective, achieve good clinical application value, and improve clinical pregnancy rate Effect

Active Publication Date: 2016-08-31
ZHEJIANG UNIV
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Problems solved by technology

[0008] In order to solve the problems of relatively subjective and poor accuracy in the existing evaluation methods, the present invention provides a relatively objective method for evaluating endometrial pinocytosis in the peri-implantation window period

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  • Method for quantification of peri-implantation window phase endometrial pinopodes
  • Method for quantification of peri-implantation window phase endometrial pinopodes
  • Method for quantification of peri-implantation window phase endometrial pinopodes

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Embodiment Construction

[0021] Further illustrate the present invention below in conjunction with accompanying drawing.

[0022] A method for quantifying endometrial pinocytosis in the peri-implantation window period of the present invention comprises the following steps:

[0023] 1. After processing the endometrial samples in the peri-implantation window period, take pictures of the endometrial epithelial cells with pinocytosis by scanning electron microscope, with a unified magnification of ×2000, and save the pictures in BMP format.

[0024] 2. Open the picture (File-open) through Image J software, and then Plugins-Analyze-Cell Counter (Plugins-Analyze-Cell Counter), Type 1 represents pinocytosis in D phase, Type 2 represents pinocytosis in FD phase, Type 3 represents pinocytosis in R phase. Record the number of pinocytic processes in the D phase, FD phase and R phase in the picture respectively, and finally calculate the number of pinocytic processes in the three phases respectively. like fig...

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Abstract

The invention finds a method for quantification of peri-implantation window phase endometrial pinopodes. The method combines scanning electron microscope images of peri-implantation window phase endometrial pinopodes with Image J software to respectively record the number of pinopodes with different developmental forms. The method mainly includes the classification mode of endometrial pinopodes' three developmental forms (developing pinopodes, fully developed pinopodes and regressing pinopodes) under a scanning electron microscope and a unique cell counting method of the scanned images in the Image J software. The method mainly solves the quantification problem of peri-implantation window phase endometrial pinopodes.

Description

Technical field: [0001] The present invention relates to a method for quantitatively evaluating endometrial pinocysts in the peri-implantation window period, a method for quantitatively evaluating endometrial pinocytic processes by combining scanning electron microscope observation of endometrial pinocytic processes with Image J software measurement method. Background technique: [0002] According to the assessment of the World Health Organization (WHO), about 1 in every 7 couples has reproductive disorders. According to a recent survey in my country, domestic infertility accounts for 10% of the number of married couples, more than double the 4.8% in the 1984 survey, and the incidence rate is on the rise. Human assisted reproductive technology (Assisted Reproductive Technology, ART) can treat infertile couples to a certain extent to achieve the purpose of fertility. It mainly includes artificial insemination, in vitro fertilization and embryo transfer (In Vitro Fertilizati...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/225
CPCG01N23/2251
Inventor 张松英刘柳赵莉娟骆东浩
Owner ZHEJIANG UNIV
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