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A yolk-free human sperm cryoprotectant solution and preservation method

A technology of cryoprotective solution and sperm, which is applied in the field of human sperm preservation, can solve the problems of inability to achieve complete sterilization, adverse reactions, and increase the interference of observation, and achieve the effects of reducing ice crystal formation and damage, clear background, and enhanced anti-oxidation Effect

Active Publication Date: 2020-12-29
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a human sperm cryoprotectant solution without egg yolk and a preservation method, which can effectively solve the problem that the existing cryoprotectant can cause adverse reactions, cannot achieve complete sterilization and increase observation interference and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A human sperm cryoprotectant solution without egg yolk, comprising the following components and the final concentration of each component when preserving human sperm: sodium chloride 90mmol / L, potassium chloride 5mmol / L, magnesium sulfate 0.2mmol / L , calcium chloride 2mmol / L, sodium dihydrogen phosphate 0.2mmol / L, sodium bicarbonate 28mmol / L, glycine 110mmol / L, 4-hydroxyethylpiperazineethanesulfonic acid 18mmol / L, glucose 5mmol / L, sucrose 30mmol / L, sodium lactate 12mmol / L, glycerin 50mL / L, and the balance is ultrapure water.

[0020] The preparation process of the human sperm cryoprotectant solution without egg yolk: accurately weigh each component with an analytical balance, namely sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, sodium dihydrogen phosphate, sodium bicarbonate, glycine, 4-Hydroxyethylpiperazineethanesulfonic acid, glucose, sucrose, sodium lactate and glycerin, and then dissolve each component in ultrapure water to make a mothe...

Embodiment 2

[0026] A human sperm cryoprotectant solution without egg yolk, comprising the following components and the final concentration of each component when preserving human sperm: sodium chloride 110mmol / L, potassium chloride 6mmol / L, magnesium sulfate 0.5mmol / L , calcium chloride 4mmol / L, sodium dihydrogen phosphate 0.5mmol / L, sodium bicarbonate 35mmol / L, glycine 150mmol / L, 4-hydroxyethylpiperazineethanesulfonic acid 25mmol / L, glucose 8mmol / L, sucrose 60mmol / L, sodium lactate 15mmol / L, glycerol 100mL / L, and the balance is ultrapure water.

[0027] The preparation process of the human sperm cryoprotectant solution without egg yolk: accurately weigh each component with an analytical balance, namely sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, sodium dihydrogen phosphate, sodium bicarbonate, glycine, 4-Hydroxyethylpiperazineethanesulfonic acid, glucose, sucrose, sodium lactate and glycerin, and then dissolve each component in ultrapure water to make a mot...

Embodiment 3

[0033] A human sperm cryoprotectant solution without egg yolk, comprising the following components and the final concentration of each component when preserving human sperm: sodium chloride 95mmol / L, potassium chloride 5.2mmol / L, magnesium sulfate 0.3mmol / L L, calcium chloride 2.5mmol / L, sodium dihydrogen phosphate 0.3mmol / L, sodium bicarbonate 30mmol / L, glycine 120mmol / L, 4-hydroxyethylpiperazineethanesulfonic acid 20mmol / L, glucose 6mmol / L, Sucrose 40mmol / L, sodium lactate 13mmol / L, glycerol 60mL / L, and the balance was ultrapure water.

[0034]The preparation process of the above-mentioned human sperm cryoprotective solution without egg yolk: accurately weigh each component with an analytical balance, namely sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, sodium dihydrogen phosphate, sodium bicarbonate, glycine, 4-Hydroxyethylpiperazine ethanesulfonic acid, glucose, sucrose, sodium lactate and glycerin, and then each component is dissolved in ultrap...

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Abstract

The invention discloses a yolk-free human sperm cryoprotectant solution and a preservation method, wherein the sperm cryoprotectant solution includes the following components and the final concentration of each component when preserving human sperm is: sodium chloride 90-110mmol / L. Potassium chloride 5-6mmol / L, magnesium sulfate 0.2-0.5mmol / L, calcium chloride 2-4mmol / L, sodium dihydrogen phosphate 0.2-0.5mmol / L, sodium bicarbonate 28-35mmol / L, glycine 110~150mmol / L, 4‑hydroxyethylpiperazineethanesulfonic acid 18~25mmol / L, glucose 5~8mmol / L, sucrose 30~60mmol / L, sodium lactate 12~15mmol / L, glycerin 50~100mL / L, The balance is ultrapure water. The cryoprotective solution for sperm does not contain yolk, will not cause allergic reactions, can have a good protective effect on the freezing damage in the process of sperm freezing, and has no obvious negative impact on the physiological function of sperm, and has good forward motility sperm after freezing recovery rate.

Description

technical field [0001] The invention belongs to the technical field of human sperm preservation, and in particular relates to a yolk-free human sperm cryoprotectant solution and a preservation method. Background technique [0002] Semen freezing technology is a technology used by human sperm banks to preserve sperm and maintain their vitality and fertilization ability. The cryoprotectant added to the semen sample in freezing technology is the key substance to protect the sperm from freezing damage during cooling and low temperature storage. Egg yolk is one of the main ingredients in the currently used cryoprotectants. However, yolk is derived from eggs and is of biological origin. Egg yolk is composed of biologically active substances such as yolk granules, phospholipids, and protein, and it is difficult to achieve complete sterilization or sterilization by filtration, high temperature, or other methods. The protein in it is a foreign protein for the human body, which may...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 岳焕勋蒋敏李福平林丽
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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