Construction method of polycystic ovarian syndrome with type 2 diabetes mellitus rat model

Abstract

The invention discloses a construction method of a polycystic ovarian syndrome (PCOS) with type 2 diabetes mellitus rat model and belongs to the technical field of medical animal models. The rat model is constructed through feeding 23-day-age weaned underage female SD (Sprague Dawley) rats with high-fat and high-sugar feed diet, subcutaneously injecting 90Mug / (g.d) sodium prasterone sulfate which is equivalent to 60Mug / (g.d) DHEA (Dehydroepiandrosterone)+0.4mL water for injection every day and continuously injecting for 20d, then fasting for 12 hours and carrying out intraperitoneal injection of STZ (Streptozocin) according to 45mg per 100mg of weight. The PCOS with type 2 diabetes mellitus model is successfully established for the first time; the construction time of the model is short and the success rate is high; a testing method is simple and feasible; the model is good in repeatability and low in cost and is an ideal PCOS with type 2 diabetes mellitus model.

Description

technical field [0001] The invention belongs to the technical field of medical animal models, and in particular relates to a method for constructing a rat model of polycystic ovary syndrome combined with type 2 diabetes. Background technique [0002] Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of childbearing age, accounting for 10%-15% of women of childbearing age. Often manifested as increased androgen, decreased or stopped ovulation, oligomenorrhea, hirsutism, acne, obesity and infertility and other symptoms. In recent years, studies have shown that PCOS patients generally have abnormal glucose and fat metabolism. About 31%-35% of PCOS patients are accompanied by abnormal glucose tolerance (IGT), and 7%-10% are accompanied by type 2 diabetes. The incidence rate is 5-10 times that of normal women, and the onset time is nearly 30 years earlier. [0003] In 2006, foreign scholars put forward the "pubertal hyperplasia theory" of PCOS wome...

AI Technical Summary

Problems solved by technology

However, it is difficult to control the dose of STZ for simple STZ injection modeling, and it is easy to establish an animal model similar to human type 1 diabetes, which makes it difficult to control the modeling rate and stability; the diabetes model induced by high-fat and high-sugar feeding combined with low-dose STZ With characteristics similar to the pathogenesis of human type 2 diabetes, it is an ideal type 2 diabetes animal model

[0006] Although the construction technology of PCOS rat model and type 2 diabetes rat model is very mature, there are no relevant reports on PCOS rat model with type 2 diabetes at home and abroad so far.

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