Kit and method for identifying cortex berberis origins
A technology of barberry bark and kit, which is applied in the field of molecular identification of Tibetan medicinal material bases, which can solve the problem that the base species cannot be used, and the diversity and complexity of bases affect the quality control and market supervision of barberry bark medicinal materials, and accurate identification and other issues, to achieve the effect of ensuring clinical efficacy, simple identification methods, and quality assurance of medicinal materials
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Embodiment 1
[0027] The DNA barcode identification of different base barberry bark of embodiment 1
[0028] 1. Experimental materials and instruments
[0029] 1 instrument
[0030] High-speed refrigerated centrifuge (CF-RXⅡ / CF-RX series, Hitachi High-Tech (Shanghai) International Trade Co., Ltd.); laboratory ultrapure water instrument system (ELGA PURELAB Ultra, Shanghai Pusong International Trade Co., Ltd.); high-pressure steam Mushroom pot (MLS-3750, Chuangbo Global (Beijing) Biotechnology Co., Ltd.); tissue grinder (SCIENTZ-48, Ningbo Xinzhi Biotechnology Co., Ltd.) WD-9413A gel imaging analyzer, DYY-8C electrophoresis instrument, DYC-33A electrophoresis tank (Beijing Liuyi Instrument Factory); micropipettes (1-2, 1-10, 2-20, 20-200, 100-1000μl, eppenorf reach plus); electronic analytical balance ( AR1530, Mettler-Toledo Instrument Co., Ltd.); acidity meter (PHS-25, Shanghai Lida Instrument Factory); WH-2 micro-vortex mixer (WH-2, Shanghai Huxi Analytical Instrument Factory); medical ...
Embodiment 2
[0079] Embodiment 2 Detection kit and detection method of the present invention
[0080] All components, content and method of use in the kit of the present invention are as follows:
[0081] 1. The composition of the kit
[0082] PCR amplification reagent (50 samples):
[0083] Including 12.5 μL of 2×Tag PCR Mix (Aidlab Biotechnologies Co., Ltd, Beijing, China), 1 μL of forward and reverse primers, 2-3 μL of DNA template, and dd H for the remaining volume 2 O Supplement. See Table 2 for PCR amplification conditions.
[0084] components
concentration
volume
Tag PCR Mix
2×
625μl
10μM
50μl
10μM
50μl
f 2 o
500μl
[0085] Standard DNA sample (50 samples)
[0086]
[0087] 2. How to use the kit
[0088] 1) DNA extraction
[0089] About 50 mg of dried berberis bark plant samples were taken and ground for 120 s at a frequency of 50 Hz with a high-throughput tissu...
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