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Kit and method for identifying cortex berberis origins

A technology of barberry bark and kit, which is applied in the field of molecular identification of Tibetan medicinal material bases, which can solve the problem that the base species cannot be used, and the diversity and complexity of bases affect the quality control and market supervision of barberry bark medicinal materials, and accurate identification and other issues, to achieve the effect of ensuring clinical efficacy, simple identification methods, and quality assurance of medicinal materials

Inactive Publication Date: 2016-11-09
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, barberry bark is a typical multi-based original Tibetan medicinal material with a wide variety of medicinal plants of the genus Berberis (Berberis L.), and the diversity and complexity of the bases seriously affect the quality of barberry bark. Control and Market Regulation
Barberry bark is a commonly used skin-like medicinal material. The appearance of each batch of medicinal materials is very similar, and the color is mostly bright yellow or yellow. The basic species cannot be accurately identified by traditional and intuitive identification methods.

Method used

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  • Kit and method for identifying cortex berberis origins
  • Kit and method for identifying cortex berberis origins
  • Kit and method for identifying cortex berberis origins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The DNA barcode identification of different base barberry bark of embodiment 1

[0028] 1. Experimental materials and instruments

[0029] 1 instrument

[0030] High-speed refrigerated centrifuge (CF-RXⅡ / CF-RX series, Hitachi High-Tech (Shanghai) International Trade Co., Ltd.); laboratory ultrapure water instrument system (ELGA PURELAB Ultra, Shanghai Pusong International Trade Co., Ltd.); high-pressure steam Mushroom pot (MLS-3750, Chuangbo Global (Beijing) Biotechnology Co., Ltd.); tissue grinder (SCIENTZ-48, Ningbo Xinzhi Biotechnology Co., Ltd.) WD-9413A gel imaging analyzer, DYY-8C electrophoresis instrument, DYC-33A electrophoresis tank (Beijing Liuyi Instrument Factory); micropipettes (1-2, 1-10, 2-20, 20-200, 100-1000μl, eppenorf reach plus); electronic analytical balance ( AR1530, Mettler-Toledo Instrument Co., Ltd.); acidity meter (PHS-25, Shanghai Lida Instrument Factory); WH-2 micro-vortex mixer (WH-2, Shanghai Huxi Analytical Instrument Factory); medical ...

Embodiment 2

[0079] Embodiment 2 Detection kit and detection method of the present invention

[0080] All components, content and method of use in the kit of the present invention are as follows:

[0081] 1. The composition of the kit

[0082] PCR amplification reagent (50 samples):

[0083] Including 12.5 μL of 2×Tag PCR Mix (Aidlab Biotechnologies Co., Ltd, Beijing, China), 1 μL of forward and reverse primers, 2-3 μL of DNA template, and dd H for the remaining volume 2 O Supplement. See Table 2 for PCR amplification conditions.

[0084] components

concentration

volume

Tag PCR Mix

2×

625μl

forward primer

10μM

50μl

reverse primer

10μM

50μl

f 2 o

500μl

[0085] Standard DNA sample (50 samples)

[0086]

[0087] 2. How to use the kit

[0088] 1) DNA extraction

[0089] About 50 mg of dried berberis bark plant samples were taken and ground for 120 s at a frequency of 50 Hz with a high-throughput tissu...

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Abstract

The invention discloses a kit for identifying cortex berberis origins. The kit contains a reagent for amplifying four gene segments containing nucleotide sequences shown as SEQIDNO: 3-6. The invention further discloses a method for identifying the cortex berberis origin. The kit and the method can effectively and accurately distinguish various origins of cortex berberis, and the identifying method is simple and stable, has the important significance on usage of standard cortex berberis and can effectively guarantee the quality of medicinal materials and a clinical curative effect, and the application prospect is good.

Description

technical field [0001] The invention relates to a molecular identification technology of the base of Tibetan medicinal materials, in particular to a kit and a method for identifying the base of barberry bark. Background technique [0002] Barberry bark is a commonly used Tibetan medicinal material, which was first recorded in "Four Medical Codes", and the Tibetan transliteration is "Jilwa", "Jiexing", "Gieerxuan" and so on. "Jingzhu Materia Medica" records: "The barberry bark is cool and rough in nature, can restrain various poisons, and dry yellow water." Barberry bark has the functions of clearing away heat, detoxifying, drying dampness, and draining yellow water, and can be used for the treatment of diseases such as dysentery, urinary tract infection, yellow water disease, and eye diseases. Modern pharmacological studies have shown that barberry bark has obvious hypoglycemic effect, and has a good protective effect on the retina of diabetic rats, and has broad applicatio...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895
Inventor 范刚何洋李艳赖先荣张艺
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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