A method for highly assisted control of cytoskeletal changes using nanotubes

Abstract

The invention discloses a method for nanotube-height-aided control of cytoskeleton change. The method comprises steps of performing anodization of pure titanium or low-modulus biphase titanium using an organic solvent; representing the microscopic structure of an anodization product using a microscopic means; preparing a titanium metal piece of nanopore and naotube microstructures; separating bone narrow mesenchymal stem cells, adhering three-generation cells on a surface-modified metal piece for incubation; observing cell adhesion effects by MTT, DYPI nuclear staining and SEM; placing the metal testing piece for cell incubation in culture holes of an aseptic elastic plate, and placing the metal testing piece in a universal testing system in a constant temperature state and providing the metal testing piece with different periods, frequencies and load compressive stress; and observing and testing cell deformation with a SEM electron microscope or optical microscope, and detecting representation of cell proliferation and differentiation. The method enables the elastic deformation signal of a modified titanium substrate surface to be amplified, thereby influencing osteoblast adhered on the modified titanium substrate surface to receive greater simulation, and leading to larger cytoskeleton change.

Description

technical field [0001] The invention relates to the field of medical biomaterials, in particular to a method for highly assisted control of cytoskeleton changes by using nanotubes. Background technique [0002] Titanium metal will undergo nonlinear elastic deformation under appropriate stress stimulation. This deformation is affected by physical elements such as elastic modulus, shape, diameter, and three-dimensional structure. Metal scaffolds produce extremely small elastic deformations; osteoblasts and stem cells are mechanical signal-sensitive or responsive cells, and studies have shown that various mechanical signals between cells and substrates can be regulated by activating specific force transduction pathways through stem cell surface receptors Differentiation of stem cells. Mechanistic factors regulate stem cell self-renewal and lineage differentiation even in the absence of biochemical stimuli. However, the elastic deformation of pure titanium metal scaffolds is v...

AI Technical Summary

Problems solved by technology

However, the elastic deformation of simple titanium metal scaffolds is very subtle, and the changes to the cytoskeleton are also very subtle, which results in a decrease in the differentiation speed and proportion of osteoblasts or stem cells, which is not conducive to the repair and replacement of various types of bone.

Images