Method for preparing oyster oligopeptide by utilizing fresh oyster meat

A technology of oyster oligopeptide and oyster meat, which is applied in food science and other fields, can solve the problems of bitter taste, difficult absorption, and large molecular weight of peptides, and achieve the effects of reducing the amount of addition, saving product costs, and increasing the added value of oysters

Inactive Publication Date: 2016-11-16
DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, oysters are used as raw materials to obtain oyster peptides through enzymatic hydrolysis. The obtained peptides h

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Grind 1000 kg of fresh oyster meat with a meat grinder for 15 minutes, and then put it into a colloid mill to obtain a slurry. Use Kjeldahl nitrogen to measure the protein content in the oyster slurry, determine the total protein mass in the oyster slurry, and then determine the amount of protein added. Enzyme amount.

[0026] (2) Introduce the slurry into 5m 3 Enzymolysis tank, the 5m 3 The enzymolysis tank is a sandwich type heating reaction kettle, add 3 times the quality of oyster slurry pure water, adjust the system temperature to 50°C, adjust the pH to 2.0 with 2.5mol / L HCL solution, add 2.5% of the total protein of the oyster slurry Composite protease; the mass ratio of composite protease is: pepsin: Aspergillus niger As3.350 protease: Usami koji 537 protease=3:4:4, stirring enzymolysis for 4h, heating up at 80°C to kill enzyme for 20min, using a tubular centrifuge Centrifuge at 16000rpm for solid-liquid separation, collect the centrifugal supernatant, and ...

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Abstract

The invention discloses a method for preparing oyster oligopeptide by utilizing fresh oyster meat. The method for preparing the oyster oligopeptide by utilizing the fresh oyster meat specially comprises the following steps: taking fresh oyster meat, and carrying out crushing and pulping so as to obtain an oyster pulp; adding water into the oyster pulp, and then, heating the mixture to 40-55 DEG C and adjusting the pH value to 2.0-5.0; adding compound proteases so as to carry out enzymatic hydrolysis, and then, raising the temperature to 80-95 DEG C so as to deactivate the enzymes; carrying out centrifugal separation, and collecting the liquid phase; separating the liquid phase by adopting nano-filtration membrane separation, and collecting the permeate liquid; performing electroosmosis on the permeate liquid so as to carry out desalination; adding beta-cyclodextrin and activated carbon into the desalinated liquid so as to carry out refining, carrying out filtrating by using a plate-and-frame filter so as to decarbonize, and collecting the liquid phase, so that a refined liquid is prepared; concentrating the refined liquid by using a membrane concentration device, and then, carrying out spray-drying, so that the oyster oligopeptide powder is prepared. The method for preparing the oyster oligopeptide by utilizing the fresh oyster meat disclosed by the invention increases added values of the oysters. The prepared products are high in oyster oligopeptide contents, and have molecular weights of 1000 Da or lower; thus, the oyster oligopeptide can be completely absorbed by the human body. The method for preparing the oyster oligopeptide by utilizing the fresh oyster meat has extremely important realistic meanings in promoting the rapid developments of the marine industry of China.

Description

technical field [0001] The invention relates to the field of oyster oligopeptide preparation. More specifically, it relates to a method for preparing oyster oligopeptides by enzymatically hydrolyzing oyster meat with fresh oyster meat. Background technique [0002] Oyster is the largest cultured shellfish in the world, and it is also one of the four famous economical shellfish cultured in my country. Oyster software is rich in protein, glycogen, taurine, amino acids and other nutrients, and has great edible and medicinal value. Peptides are intermediate products of protein hydrolysis, generally, those consisting of 2 to 10 amino acids are called oligopeptides, and those consisting of 11 to 50 amino acids are called polypeptides. In terms of material composition, peptides have homogeneity with proteins, but have unique physiological functions different from amino acids and proteins, especially oligopeptides. Scientific research shows that the main form of protein absorbed ...

Claims

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Application Information

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IPC IPC(8): A23L17/20
Inventor 包卫洋马普王祖哲杨美圆
Owner DALIAN SHENLAN PEPTIDE TECH R & D CO LTD
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