Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vermicularis yellow y28 and its application in the control of fruit tree rot

A technology for Demodex yellow and rot disease, which is applied to Demodex yellow Y28 and its application in the prevention and control of fruit tree rot, can solve the problems of pathogenic bacteria such as drug resistance, and achieve the effect of reducing adverse effects

Active Publication Date: 2019-12-20
ANHUI AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past, the prevention and treatment of pear tree rot mainly relied on thiram and other chemicals, but thiram has been banned from being used on fruit trees
At the same time, chemical fungicides are limited due to the tendency of bacteria to develop drug resistance and consumers' increasing concerns about human health, environmental pollution and chemical residues in agricultural products. Therefore, it is urgent to find a new, safe and non-toxic method or means Alternatives to chemical fungicides to control pear tree rot

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vermicularis yellow y28 and its application in the control of fruit tree rot
  • Vermicularis yellow y28 and its application in the control of fruit tree rot
  • Vermicularis yellow y28 and its application in the control of fruit tree rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening and Identification of Endophytic Fungus Y28

[0037] 1. Isolation, purification and screening of endophytic fungus Y28

[0038] 1.1 Isolation of endophytic fungi from leaves and branches (50 samples each)

[0039] The collected samples (the samples are from Hefei High-tech Agricultural Park Fruit Tree Practice Base 'Qiubai' and other pear varieties) were first washed with flowing tap water to remove surface impurities, and then surfaced in an ultra-clean workbench. Disinfection: Rinse the surface with sterile water, absorb the water with sterile filter paper, soak the tissue in 15% sodium hypochlorite solution for 5-10 seconds, and then rinse with sterile water for 3 times. The sterilized tissue was cut into the inside of the plant tissue with a scalpel, and the internal tissue was cut into small squares of about 5mm×5mm, placed on a sterile PDA for cultivation and numbered.

[0040] 1.2 Purification of endophytic fungi

[0041] Place the inoculat...

Embodiment 2

[0065] Example 2 Application of bacterial strain Y28 in the prevention and treatment of pear tree rot

[0066] Collect ascospores of bacterial strain Y28 and use sterile water to prepare a concentration of 10 8 CFU / mL spore suspension, apply the prepared spore suspension to the rot wound that has been scraped off completely, the scraped area exceeds 0.5cm-1.0cm of the lesion, and then apply the spore suspension. The results showed that the rot disease scars coated with spore suspension healed well, and the diseased tissues were obviously repaired ( Figure 4 ).

[0067] It can be seen that the strain Y28 has the characteristics of safe and efficient prevention and control of rot disease, and this strain is a potential excellent strain for the prevention and control of rot disease.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an endophytic fungus-Demodex yellow Y28 with biocontrol effect, which is isolated from a pear tree variety with high resistance to rot, and the preservation number of the strain Y28 is CGMCC No.11918. The invention also provides the application of Demodex chrysogenum Y28 in preventing and treating fruit tree rot. The test showed that the antibacterial rate of strain Y28 reached 70.50% in the in vitro confrontation bacteriostasis test, and 55.54% in the in vitro rescreening test of annual branches. It is a better biocontrol strain for preventing and treating pear tree rot .

Description

technical field [0001] The invention relates to the technical field of microbiology and biological control, in particular to Demodex chrysogenum Y28 and its application in preventing and controlling fruit tree rot. Background technique [0002] Pear trees are the third largest fruit tree species in my country. The healthy development of the pear industry not only plays an important role in my country's agricultural production and farmers' income increase, but also has a decisive impact on the world's pear fruit production. [0003] The pathogenic bacterium of pear tree rot disease is pear rot fungus, which belongs to Ascomycota subphylum fungus, apple black rot shell pear variety (Valsapyri), and the morphology of the pathogen is similar to that of apple tree rot fungus. Pear tree rot occurs in all major pear producing areas in my country, especially in Xinjiang, Northwest, North China and other places. According to the survey, the rate of diseased strains of fragrant pears...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 刘普周珊珊汤小美朱立武叶振风贾兵衡伟刘莉
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com