Production method of reduced coenzyme q10

A production method and reduction technology, which are applied in the production field of reduced coenzyme Q10, can solve the problems of oxidation of reduced coenzyme Q1 and the like, and achieve the effect of stable properties

Active Publication Date: 2020-12-29
米涛(上海)环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since reduced coenzyme Q1 is easily oxidized by molecular oxygen, there is no report on the technology for producing reduced coenzyme Q10 on an industrial scale.

Method used

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  • Production method of reduced coenzyme q10
  • Production method of reduced coenzyme q10
  • Production method of reduced coenzyme q10

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]The production method of reduced coenzyme Q10 includes the following steps:

[0036](1) The human liver cell line Fa2N-4 (ATCC-5566) was selected as the parent material for the production of reduced coenzyme Q10.

[0037](2) Take 2.2L of DMEM culture medium (using the DMEM (high sugar) product number CM0103 provided by Youkang Hengye Biotechnology (Beijing) Co., Ltd. containing L-glutamine, without sodium pyruvate cell culture medium) in 121 Sterilize at ℃ for 10 minutes, add 10% of the volume of the culture solution to produce reduced coenzyme Q10 human liver cell line Fa2N-4 (ATCC-5566), at a temperature of 37 ℃, CO2In an environment with a volume fraction of 5% and a stirring rotation speed of 300 rpm for 48 hours, the cell liquid was obtained.

[0038](3) Add 30 mL of acid solution to the culture solution, stir and mix uniformly, and then centrifuge at 3000 rpm for 10 minutes to collect the wet cell-containing body, and wash it with deionized water three times to obtain 198 g of wa...

Embodiment 2

[0046]It is basically the same as Example 1, except that the acid is obtained by mixing and mixing fumaric acid and succinic acid at a molar ratio of 1:1. The reduced coenzyme Q10 of Example 2 was obtained.

Embodiment 3

[0048]It is basically the same as Example 1, except that the acid is obtained by mixing oxaloacetic acid and succinic acid at a molar ratio of 1:1. The reduced coenzyme Q10 of Example 3 was obtained.

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Abstract

The invention discloses a method for producing reduced coenzyme Q10. The method comprises the following steps that 1, the cell line or cell strain of a person is selected as a female parent material for producing the reduced coenzyme Q10; 2, a DMEM culture solution is taken, stirring culture is carried out for 24 hours to 72 hours, and cell sap is obtained; 3, acid liquor is added into the culture solution, the mixture is stirred and mixed to be uniform, then centrifugal separation is carried out, a cell containing wet object is collected, and after washing is carried out 2-4 times with water, cleaned cyton is obtained; 4, water is added into the cleaned cyton so that the cyton is pasty, and the dried cyton is obtained after spray-drying treatment; 5, an organic solvent is added into the dried cyton, filtering is carried out, and an extraction solution is obtained; 6, the extraction solution is cooled to 4 DEG C, and by means of the method for producing the reduced coenzyme Q10, the reduced coenzyme Q1 is obtained through separating out and drying under reduced pressure. Compared with an existing method, no alkaline matter is added in the preparation process, the property is stable, the coenzyme Q10 cannot be oxidized by oxygen in air, and the method for producing the reduced coenzyme Q10 is high in quality.

Description

Technical field[0001]The invention relates to a reduced coenzyme Q10, in particular to a production method of reduced coenzyme Q10.Background technique[0002]Since Coenzyme Q10 was discovered by scientists in the middle of last century and confirmed its chemical structure, its important role in human physiology and life processes has also been gradually recognized by us.[0003]Coenzyme Q10, also known as ubiquinone 10, is a fat-soluble quinone. It is orange-yellow crystals at room temperature. It has a melting point of 48°C, odorless and tasteless. Its structure is similar to vitamin K because of the side chain at the sixth position of the core ( The polyisoprenyl has a degree of polymerization of 10 and named after it) is a quinone compound. Another form of it is a reduced coenzyme, whose physiological functions are mainly derived from the redox properties of the quinone group and the physical properties of the isoprenoid side chain.[0004]With the deepening of human life science rese...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12P7/66C07C46/10C07C50/28
CPCC07C46/10C12P7/66C07C50/28
Inventor张启明张莉
Owner米涛(上海)环保科技有限公司