Reduced coenzymes Q10 and cosmetics

A reduced and cosmetic technology, applied in the field of reduced coenzyme Q10, can solve problems such as the oxidation of reduced coenzyme Q10, and achieve the effect of stable properties

Active Publication Date: 2017-03-15
广东润和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because reduced coenzyme Q10 is easily oxidized by molecular oxygen, there are no reports on the technology of reducing coenzyme Q10 on an industrial scale.

Method used

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  • Reduced coenzymes Q10 and cosmetics
  • Reduced coenzymes Q10 and cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of reduced coenzyme Q10:

[0033] (1) The human liver cell line Fa2N-4 (ATCC-5566) was selected as the parent material for the production of reduced coenzyme Q10.

[0034] (2) Take 2.2L of DMEM culture medium (using the DMEM (high sugar) containing L-glutamine and excluding sodium pyruvate cell culture medium provided by Youkang Hengye Biotechnology (Beijing) Co., Ltd. with the article number CM0103) at 121 Sterilize at ℃ for 10 minutes, add human liver cell line Fa2N-4 (ATCC-5566) that accounts for 10% of the volume of the culture medium, and produce reduced coenzyme Q10 at a temperature of 37 ℃, CO 2 In an environment with a volume fraction of 5% and a stirring speed of 300 rpm, the cells were cultured for 48 hours to obtain cell fluid.

[0035] (3) Add 30mL of acid solution to the culture medium and stir to mix evenly. Use Mulitibeads shocker B300 to oscillate and stir for 1 hour to break up the cell bodies, then centrifuge at 3000 rpm for 10 minutes to ...

Embodiment 2

[0043] It is basically the same as in Example 1, except that the acid is obtained by stirring and mixing uniformly citric acid and L-malic acid in a molar ratio of 1:1. The reduced coenzyme Q10 of Example 2 was obtained.

Embodiment 3

[0045] It is basically the same as in Example 1, except that the acid is obtained by stirring and mixing L-ascorbic acid and L-malic acid in a molar ratio of 1:1. The reduced coenzyme Q10 of Example 3 was obtained.

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Abstract

The invention discloses reduced coenzymes Q10 and cosmetics. According to the reduced coenzymes Q10, a cell line or a cell strain of the human serves as a female parent for producing the reduced coenzymes Q10, cell culture is carried out in a biological culture agent DMEM culture solution, after cell sap containing a large number of reduced coenzymes Q10 is obtained, acid liquor adding treatment, centrifugal separation, spray drying, organic solvent extraction and concentration, and cooling crystallization are carried out, and the reduced coenzymes Q10 are obtained. Compared with existing disclosed reduced coenzymes Q10, according to the reduced coenzymes Q10, no alkaline matter is added in the preparation process, properties are stable, and the reduced coenzymes Q10 cannot be oxidized by oxygen in air; no residues of a reducing agent exist, no antioxidant or protective agent or other chemical residues exist, and the reduced coenzymes Q10 are high in quality; the reduced coenzymes Q10 can be used for food, medicine and the cosmetics, or can be used for injection solutions and infusion, and have no immunogenic reaction on the human body.

Description

technical field [0001] The invention relates to a reduced coenzyme Q10, in particular to a reduced coenzyme Q10 and cosmetics. Background technique [0002] Since coenzyme Q10 was discovered by scientists in the middle of the last century and its chemical structure was confirmed, its important role in human physiology and life processes has been gradually recognized by us. [0003] Coenzyme Q10, also known as ubiquinone 10, is a fat-soluble quinone. It is an orange-yellow crystal at room temperature, with a melting point of 48°C. It is odorless and tasteless. Its structure is similar to vitamin K. Polyisoprenyl is named after its degree of polymerization is 10) is a quinone compound. Another form of it is a reduced coenzyme, and its physiological functions are mainly derived from the redox properties of the quinone group and the physical properties of the isoprenoid side chain. [0004] With the deepening of human life science research, since Harman put forward the free ra...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12P7/66C07C46/10C07C50/28A61K8/35A61Q19/00
CPCA61K8/355A61K2800/10A61Q19/00C07C46/10C12P7/66C07C50/28
Inventor张启明张莉
Owner广东润和生物科技有限公司