A protein-mediated method for synthesizing and modifying cerium nanomaterials

A nanomaterial and protein technology, applied in nanotechnology, metal processing equipment, transportation and packaging, etc., can solve the problems of synthetically modified cerium nanoparticles and cerium nanochains, and achieve stability and biocompatibility guarantee, Good biocompatibility and far-reaching effects

Inactive Publication Date: 2018-02-09
中国人民解放军第三军医大学军事预防医学院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason why BSA can participate in the synthesis and modification of various metal nanomaterials is mainly because there is a certain interaction between itself and metal ions or nanomaterials. There is a redox reaction between metallothionein (Chem.Res.Toxicol.2015, 28, 2304-2312), but there is no literature report on the modification of a single cerium nanocluster by serum protein molecule-mediated synthesis, let alone Any literature reports based on serum proteinoid-mediated synthesis of modified cerium nanoparticles and cerium nanochains

Method used

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  • A protein-mediated method for synthesizing and modifying cerium nanomaterials
  • A protein-mediated method for synthesizing and modifying cerium nanomaterials
  • A protein-mediated method for synthesizing and modifying cerium nanomaterials

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Preparation of cerium nanoclusters

[0034] Take 0.25g BSA and add it into 9mL distilled aqueous solution, take 500uL 0.1mol / L Ce(NO 3 ) 3 .6H 2 Add 0 L to it, and under 600r / min magnetic stirring, react at room temperature (37°C) for 15 minutes, and then the system becomes a little turbid. Take 500uL 1mol / L KOH and add it to the above system to adjust the pH to about 13, and react at room temperature (37°C) for 15 minutes. The reaction was stopped, and the above reaction solution was placed in a dialysis bag with a molecular weight cut off of 10,000 and dialyzed with twice distilled water for 24 hours, wherein the water was changed every 6 hours, and the water change volume was 3 L / time. To remove unreacted cerium salt, hydroxide (OH - ) and other inorganic ions. Finally, place in a 100kd (15mL) sleeve centrifuge tube and centrifuge at 4500r / min for 5 times, remove excess unreacted BSA, and obtain ceria nanoclusters (ceria nanoclusters, 8mL, C Ce =0.55mg...

Embodiment 2

[0035] Example 2 Preparation of cerium nanoparticles

[0036] Take 0.25g BSA and add it to 9mL distilled aqueous solution, place it in an oil bath at 80°C for 10min, and then add 500uL 0.1mol / L Ce(NO 3 ) 3 .6H 2 O solution, the system was immediately turbid, and 500uL 1mol / L KOH was added to the above system at one time to adjust the pH to about 13. After 5 minutes of reaction in an oil bath at 80°C, the system was clear (light yellow), and the reaction was stopped after continuing to control the temperature for 2 hours. Cool to After 25°C, place the above reaction solution into a dialysis bag with a molecular weight cut-off of 10,000 for dialysis for 24 hours, and change the water every 6 hours at a rate of 3 L / time. To remove unreacted cerium salt, hydroxide (OH - ) and other inorganic ions. Finally placed in a 100kd (15mL) sleeve centrifuge tube, 4500r / min centrifugal washing 5 times, remove excess unreacted BSA, to obtain ceria nanoparticles (ceria nanoparticles, 8mL, ...

Embodiment 3

[0037] Example 3 Preparation of cerium nanochains (short chain ~ 70nm, DLS)

[0038] Take 0.25g BSA and add it into 9mL distilled aqueous solution, take 500uL 0.1mol / L Ce(NO 3 ) 3 .6H 2 Add 0 L to it, under 600r / min magnetic stirring, react at room temperature (37°C) for 15 minutes, and then the system is slightly turbid. Take 500uL 1mol / L KOH and add it to the above system to adjust the pH to about 13. After reacting at room temperature (37°C) for 4 hours The reaction was stopped, and the above reaction solution was placed in a dialysis bag with a molecular weight cut off of 10,000 and dialyzed with twice distilled water for 24 hours, wherein the water was changed every 6 hours, and the water change volume was 3 L / time. To remove unreacted cerium salt, hydroxide (OH - ) and other inorganic ions. Finally placed in a 100kd (15mL) sleeve centrifuge tube, 4500r / min centrifugal washing 5 times, remove excess unreacted BSA, to obtain ceria nanochains (ceria nanochains, 8mL, C ...

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Abstract

The invention relates to a method for protein-mediated synthesis and modification of cerium nanomaterials. Firstly, a certain concentration of serum protein and cerium nitrate are mixed to form a precursor solution of cerium nanomaterials, which are nucleated and grown under alkaline conditions of potassium hydroxide solution. By further adjusting the reaction temperature and time, on the one hand, cerium nanoclusters, cerium nanoparticles and cerium nanochain materials are controllably obtained, and all have good uniformity, dispersion and stability. The nanomaterials and their surfaces were characterized and analyzed. On the other hand, the BSA or HSA modified on the surface of the corresponding cerium nanomaterials has excellent biocompatibility, and has a large number of functional groups available for modification, such as carboxyl, amino etc., and other functional molecules can be further grafted and modified, laying the foundation for its further application in vivo. The invention reflects green economy, flexible and easy-to-control regulation mode, and strong repeatability.

Description

technical field [0001] The invention relates to a method for protein-mediated synthesis and modification of cerium nanomaterials, which belongs to the technical field of preparation of surface-modified functional nanomaterials. Specifically. It involves a new method of using serum protein biomacromolecules to mediate the synthesis and modification of cerium nanomaterials under alkaline conditions, especially involving the green controllable synthesis and modification of cerium nanoclusters, cerium nanoparticles and cerium nanochains. Background technique [0002] Ce nanomaterials due to their surface Ce 3+ / C 4+ Coexistence endows it with excellent redox activity, which makes it have very important and broad application prospects in chemical catalytic reaction, radiation protection, tumor radiotherapy and chemotherapy, treatment of antioxidant diseases and regenerative medicine (Nanoscale.2011, 3, 1411-1420, NPG Asia Mater. 2014, 6, e90, ACSNano. 2016, 10, 2860–2870, Chem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB82Y40/00B22F9/24B22F1/054
Inventor 李蓉阳章友龙爽罗圣霖曾一平史春梦王卫东
Owner 中国人民解放军第三军医大学军事预防医学院
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