Typhoid bacillus colloidal gold method detection kit

A detection kit and typhoid bacillus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of atypical clinical symptoms, easy misdiagnosis, and low fatality rate, and achieve high sensitivity and specificity, rapid detection, Quick and easy effects

Inactive Publication Date: 2017-01-04
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The case fatality rate is not high, the clinical symptoms are often atypical, and it is easy to misdiagnose

Method used

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  • Typhoid bacillus colloidal gold method detection kit
  • Typhoid bacillus colloidal gold method detection kit
  • Typhoid bacillus colloidal gold method detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of embodiment 1 test paper card of the present invention

[0047] 1) Use the pretreatment buffer to pretreat the gold standard pad. The pretreatment buffer is: fructose 1.5g / L, aluminum hydroxide 0.5g / L, glucose 1.25g / L, sodium chloride 0.3g / L, glycine 2.0g / L aqueous solution, pH = 7.4, the specific steps of pretreatment are: soak the gold standard pad in the pretreatment solution for 2 hours, take it out and dry it at 37°C; then use colloidal gold-labeled recombinant Salmonella typhi antigen and mouse Spray the pretreated gold standard pad with IgG solution to prepare a gold standard pad coated with recombinant Salmonella typhi antigen and mouse IgG. The mass ratio of colloidal gold to antibody in the solution is 5:1, and the concentration of the solution is 10mg / ml. The amount is 4ul / cm;

[0048] 2) Spray 1 mg / ml of recombinant Salmonella typhi antigen solution and goat anti-mouse antibody solution on the detection line and quality control line of the...

Embodiment 2

[0050] The preparation of embodiment 2 comparison kit

Embodiment 3

[0055] The specificity experiment of embodiment 3 detection kits

[0056] Detection method:

[0057] 1. Get the kit of the present invention prepared in Example 1 and the comparative kit of Example 2, and place the kit on a horizontal platform.

[0058] 2. Preparation of samples to be tested: according to the four types of test objects in Table 1, serum or plasma was taken as samples to be tested.

[0059] 3. There are 100 subjects for each type of experiment, and the test results are shown in Table 1:

[0060] Table 1 Test kit specificity test results

[0061]

[0062]

[0063]

[0064] It can be seen from the above table that the detection kit of the present invention has no cross-reaction to hepatitis B and syphilis, and has good specificity.

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Abstract

The invention relates to the field of biological detection, and specifically relates to a typhoid bacillus colloidal gold method detection kit, and a preparation method and application thereof. The invention provides the typhoid bacillus colloidal gold method detection kit, which comprises a test paper card. The test paper card comprises a bottom plate, and a sample pad, a gold label pad, a nitrocellulose membrane and an absorbent pad which are sequentially arrayed from a sample adding end on the surface of the bottom plate; a recombined typhoid bacillus antigen and rat IgG are contained on the gold label pad; the nitrocellulose membrane is coated with detection lines and quality control lines; the recombined typhoid bacillus antigen and the rat IgG on the gold label pad adopt colloidal gold to label. The typhoid bacillus colloidal gold method detection kit provided by the invention is used for detecting typhoid bacillus through a colloidal gold immunochromatography technique for the first time, has both sensitivity and specificity, and has the advantages of quickness, simplicity and convenience in operation, accuracy in result, economic benefit, applicability and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a colloidal gold detection kit for Salmonella typhi and its preparation method and application. Background technique [0002] Typhoid fever is an acute intestinal infectious disease transmitted through the digestive tract caused by Salmonella typhi. The clinical features are long-term fever, systemic poisoning symptoms, relative bradycardia (pulse is not proportional to body temperature), hepatosplenomegaly, skin roseola, and leukopenia. The main complications were intestinal bleeding and intestinal perforation. The source of infection is patients and bacteria. It can occur throughout the year, but it is more common in summer and autumn. July-October is the high incidence season of typhoid fever, which is related to people's preference for raw and cold food and frequent fly activities in summer and autumn. It is contagious throughout the whole process, and the infectivity i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/558G01N33/56916Y02A50/30
Inventor 丁晓辉毛远丽
Owner SHANGHAI CHEMTRON BIOTECH
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