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Micro-droplet reaction coding detection method and system

A coding detection and micro-droplet technology, which is applied to chemical instruments and methods, instruments, laboratory utensils, etc., can solve problems such as complexity and limitation of coding capabilities, and achieve the effect of large coding capacity and easy integration into lab-on-a-chip

Active Publication Date: 2018-08-24
TARGETINGONE TECH (BEIJING) CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The encoding method based on nucleic acid sequence has a high encoding capacity, but because nucleic acid sequence sequencing requires a relatively complicated process, this encoding method is only suitable for some specific occasions
[0008] Due to the limitation of the coding ability of existing methods, the current multi-index digital PCR can only realize the simultaneous detection of several to dozens of different sites

Method used

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  • Micro-droplet reaction coding detection method and system

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1 Micro-droplet reaction coding principle of the present invention

[0043] Such as figure 1 As shown, by controlling the working order of each external syringe pump, different reagents can generate micro-droplets in the microfluidic chip pipeline in a specific order, and arrange them in a queue in this order. Micro-droplets with label information become marker droplets, and the area in the queue is called the coding area; micro-droplets with biochemical reaction reagents become functional droplets, and the area in the queue is called the functional area. There is a coding region between the functional regions. The complete coding "queue" is formed by the repeating structure of the reaction region plus the coding region. When the coding "queue" enters the detection area, according to the order of sample injection and the information of each coding area, the reactants corresponding to the functional droplets contained in each functional area can be decoded. ...

Embodiment 2

[0044] Embodiment 2 Micro-droplet reaction coding detection system of the present invention

[0045] figure 2 It is a schematic diagram of the micro-droplet reaction coding detection system of the present invention, and the system is divided into four areas: sample injection area, droplet generation area, reaction area and detection area. A1, A2...An are corresponding to different injection ports, which are injected with a syringe pump. A1 is the injection port for generating marker droplets, A2, A3...An is the injection port for generating functional droplets. When the whole system is working, the order of injection is A1-A2-A1-A3 -A1...that is, the functional droplets are separated by marker droplets when injecting samples. At the intersection point connected by B1 and B2 (that is, the droplet generation area), after the droplets are generated by the water-in-oil method, they are arranged in the reaction area in a certain sequence and certain biochemical reactions occur. ...

Embodiment 3

[0046] Example 3 Using high fluorescence intensity as the encoding of the characteristics of the marker droplet to realize multi-site gene detection

[0047] Step 1 Liquid reagent injection control

[0048] The encoding method proposed by the present invention requires droplets to form a "queue" in which the coding area and the functional area are interlaced in the pipeline, so there are certain requirements for the order of different reagent injections, and the order of injection is controlled by the external syringe pump. The order of work is determined.

[0049] figure 2 The syringe pump shown is a high-precision syringe pump (Harvard Apparatus, PHD Ultra). Each syringe pump is fixed with a 1ml syringe and connected to the chip inlet through a Teflon tube. Each syringe is loaded with different reagents, among which the syringes corresponding to B1 and B2 are loaded with mineral oil for subsequent droplet generation; the syringe corresponding to A1 is loaded with M at a c...

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Abstract

The invention provides a micro-droplet reaction encoding detection method and system. The encoding detection method comprises the following steps that different reagents are used for generating micro-droplets in a micro-fluidic chip pipeline in sequence, and the micro-droplets are arranged into a queue according to the sequence; the micro-droplets with marker information become marked droplets, and the region where the queues are located become the encoding regions; the micro-droplets with a biochemical reaction reagent become functional droplets, and the regions where the queue are named as the functional regions; one encoding region is arranged between every two adjacent functional regions in a spaced mode, and a complete encoding queue is formed by the repeated structures of the reaction regions and the functional regions. The encoding capacity is large and is not limited by the encoding method, encoding substances and biochemical reactions are completely isolated in space, encoding is easy, the encoding robustness is high, and a chip laboratory is easy to integrate.

Description

technical field [0001] The invention relates to the field of micro-droplet biomedical detection, in particular to a micro-droplet reaction coding detection method and a system thereof. Background technique [0002] Droplet microfluidic technology is an important part of microfluidic core technology, which is characterized in that two incompatible fluids are formed into micron-scale droplets in microchannels or capillaries. The most common in the field of biomedical testing is the "water-in-oil" droplet. Among them, oil acts as a carrier that isolates each droplet from each other. Such micron-scale droplets are also used as microreactors. That is to say, a droplet with a diameter of tens of microns can be regarded as a test tube used in traditional biochemical detection, and it can also perform biochemical reactions in micro-droplets and be used for the detection of target molecules in biomedicine. Compared with traditional test tubes, the advantages of the micro-droplet r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N35/00
CPCB01L3/527B01L2200/10B01L2300/02B01L2300/0867G01N35/00732G01N2035/00772
Inventor 苏世圣郭永荆高山王博张妙琪柳哲高华方祝令香杨文军王勇斗
Owner TARGETINGONE TECH (BEIJING) CORP
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