Culture method for reducing pollution rate of transgenic tobacco seedlings and promoting rapid rooting of transgenic tobacco seedlings
A technology of transgenic seedlings and cultivation methods, which is applied in the field of tobacco transgenics, can solve the problems of long cycle and few transformants, and achieve the effects of shortening the transformation cycle, reducing the pollution rate, and improving the transformation efficiency
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[0022] A cultivation method for reducing the pollution rate of tobacco transgenic seedlings and promoting their rapid rooting, comprising the steps of:
[0023] (1), preparation and infection of explants: take the leaves of Nicotiana benthamiana in good growth state, avoid the main veins and leaf edges, cut into small pieces of 1cm*1cm, and place them on MS medium for pre-cultivation for 2-3 days . Put the Agrobacterium suspension infection solution containing 200μmol / L acetosyringone into the infection for 20 minutes, shake the culture dish once every 5 minutes during the process, then pour out the bacterial solution, and dry the explants with sterilized absorbent paper The surface bacterial liquid is transferred to the solid co-culture medium (the formula of the solid co-culture medium is MS macroelement, MS trace element, MS iron salt, 0.5mg / L thiamine hydrochloride, 0.2mg / L pyridoxine hydrochloride, 0.2mg / L niacin, 150 mg / L inositol, 200 μmol / L acetosyringone, 1 mg / L nap...
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