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Application of lncRNA-LOC100507195 in mycobacterium tuberculosis negative pulmonary tuberculosis diagnosis

A technology for pulmonary tuberculosis and tuberculosis, which is applied in the application field of lncRNA‑LOC100507195 in the diagnosis of bacterial-negative pulmonary tuberculosis

Pending Publication Date: 2017-02-01
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the detection of pulmonary tuberculosis using lncRNA as a diagnostic marker

Method used

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  • Application of lncRNA-LOC100507195 in mycobacterium tuberculosis negative pulmonary tuberculosis diagnosis
  • Application of lncRNA-LOC100507195 in mycobacterium tuberculosis negative pulmonary tuberculosis diagnosis
  • Application of lncRNA-LOC100507195 in mycobacterium tuberculosis negative pulmonary tuberculosis diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Up-regulated expression of LOC100507195 in MΦ infected with MTB

[0024] Isolation of PBMC from healthy volunteers, immunomagnetic separation of CD14 + For monocytes, GM-CSF was used to induce monocytes to differentiate into MΦ, and the obtained MΦ was infected with MTB. After 72h, real-time fluorescent quantitative PCR was used to detect the expression level of LOC100507195 in MΦ.

[0025] Test results such as figure 1 As shown, it can be seen that the expression level of LOC100507195 in MΦ after MTB infection is significantly up-regulated, indicating that MTB infection promotes the expression of LOC100507195.

Embodiment 2

[0026] Example 2 Detection of expression of LOC100507195 in PBMCs of patients with bacterial negative pulmonary tuberculosis by real-time fluorescent quantitative PCR

[0027] The RNA of PBMC of 30 patients with bacterium-negative pulmonary tuberculosis and 32 healthy volunteers was extracted, and the expression of lncRNA-LOC100507195 was detected by real-time fluorescent quantitative PCR.

[0028] Test results such as figure 2 As shown, it can be seen that the expression of LOC100507195 in PBMCs of patients with bacterial negative pulmonary tuberculosis showed a significant down-regulation trend (P<0.001).

Embodiment 3

[0029] Example 3 LOC100507195 as a new target for the diagnosis of bacterial negative pulmonary tuberculosis

[0030] This study found that compared with healthy people, the expression of LOC100507195 in PBMCs of tuberculosis patients showed a very significant down-regulation trend (P<0.001). In order to understand whether the expression change of LOC100507195 in PBMC can be used to distinguish healthy people from tuberculosis patients, we used ROC curve to analyze whether the expression change of LOC100507195 in PBMC is used for the diagnosis of tuberculosis.

[0031] According to the relative expression of LOC100507195 in PBMCs of healthy people and tuberculosis patients, statistical analysis software was used to establish a ROC curve with specificity on the abscissa and sensitivity on the ordinate, see image 3 . The results showed that the area under the ROC curve AUC(A)=0.8594, indicating that LOC100507195 has a high accuracy in the diagnosis of tuberculosis patients ( ...

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Abstract

The invention discloses application of lncRNA-LOC100507195 in mycobacterium tuberculosis negative pulmonary tuberculosis diagnosis. It is found that after MTB is infected with macrophage, consequently LOC100507195 expression is up-regulated. The detection results on the PBMC of 30 mycobacterium tuberculosis negative pulmonary tuberculosis patients show that the expression level of LOC100507195 is remarkably lower than that of a healthy volunteer; LOC100507195 is possibly adopted as an antituberculous protective factor of the macrophage, the expression of LOC100507195 is down-regulated, and consequently the body is likely to be infected with tuberculosis. The receiver operating characteristic curve analysis result shows that LOC100507195 is expected to become a new target spot of mycobacterium tuberculosis negative pulmonary tuberculosis diagnosis. Uninfected people and the mycobacterium tuberculosis negative pulmonary tuberculosis patients can be effectively distinguished by detecting the expression level of LOC100507195.

Description

technical field [0001] The present invention specifically relates to the application of lncRNA-LOC100507195 in the diagnosis of bacterial negative pulmonary tuberculosis. Background technique [0002] Tuberculosis is still the infectious disease with the highest infection rate in the world, and the prevention and control situation is very severe. Rapid and sensitive diagnosis is the key to improving the treatment effect and effectively controlling the spread of tuberculosis. Among the pulmonary tuberculosis patients in China, bacterium-negative pulmonary tuberculosis patients account for 60% to 70% of active pulmonary tuberculosis patients, and the clinical symptoms and imaging features of many other lung diseases are difficult to distinguish from tuberculosis. There is a lack of effective diagnostic methods for bacterial-negative pulmonary tuberculosis, which often results in delayed or misdiagnosed patients. Although my country has formulated some diagnostic criteria, th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 马骊杨晓帆温茜
Owner SOUTHERN MEDICAL UNIVERSITY