Method for quickly identifying beef

Abstract

The invention provides a method for quickly identifying beef, and relates to the technical fields of food detection and animal molecular biology. The method comprises the following steps: preparing 10 mu L of PCR (polymerase chain reaction) reaction system by designing one group of primers for specifically recognizing sheep mitochondrial genes and regulating the total DNA (deoxyribonucleic acid) concentration of a sample purified through a phenol-chloroform extraction method to 20 ng / mu L to serve as a PCR detection template; carrying out a reaction program of denaturing at 98 DEG C for 10 seconds, annealing at 63 DEG C for 5 seconds and performing extension reaction at 72 DEG C for 5 seconds; performing 30 cycles for PCR implementation; detecting a PCR product through 1.2% agarose gel electrophoresis; and based on the electrophoresis detection result, indicating that the sample is beef or contains bovine-derived ingredients if a 162bp PCR product exists. The method provided by the invention can quickly and accurately identify whether a sample is mutton or whether mutton ingredients are contained in a sample, and can also be directly used for producing a kit for identifying mutton.

Description

technical field [0001] The invention belongs to the technical field of food detection and animal molecular biology, relates to a method for rapidly identifying beef, and is suitable for identifying whether an unknown sample is beef or whether it contains beef components. Background technique [0002] With the improvement of the living standard of our country's residents, only the domestically produced meat is far from meeting the market demand, and a part of meat needs to be imported from abroad. These factors have created conditions for illegal traders at home and abroad to take advantage of the situation. In order to protect the legitimate rights and interests of consumers, it is necessary to establish an effective identification method for meat-derived ingredients. [0003] PCR (polymerase chain reaction), that is, DNA polymerase chain reaction, is a molecular biology technique invented in the 1980s that integrates high specificity, sensitivity, and high efficiency. It i...

AI Technical Summary

Problems solved by technology

[0004] In practical work Taq Both traditional PCR and real-time quantitative PCR of DNA polymerase are used. The former usually takes a long time, while the latter has the problem of expensive reagents, consumables and detection equipment although the detection time is short.

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