Method of extracting and detecting mogrosides IV and V from momordica grosvenori of 55 days in fruiting age
A technique of extracting mogroside from grosvenoria grosvenori, applied in the field of extraction and detection of mogroside, can solve the problems of not extracting mogroside IV, and achieve the effect of novel combination technology
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[0041] Synchronous extraction method of high-content mogroside IV and mogroside V extracts:
[0042] 1. Take 2 fresh grosvenor fruits with a fruit age of 55 days, wipe them clean, weigh them and make a record, mash them with a masher to obtain grosvenor pulp; add 60% ethanol aqueous solution at a solid-to-liquid ratio of 1:2, and place in an ultrasonic instrument middle. Ultrasonic conditions: ultrasonic instrument frequency 53HZ, power 100W, temperature 50°C, take out after ultrasonic for 30min, filter with gauze after cooling, take the filtrate, extract the filter residue three times in the same way, combine the filtrate three times, and set aside.
[0043] Add 10% Ca(OH) of 15% by weight of Luo Han Guo pulp 2 Suspended liquid, and stirring, at this time, it will be found that the Luo Han Guo extract obviously has the phenomenon of macromolecular substance aggregation. After the temperature drops to room temperature, put the beaker containing the Luo Han Guo solution into t...
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