Method of extracting and detecting mogrosides IV and V from momordica grosvenori of 55 days in fruiting age

A technique of extracting mogroside from grosvenoria grosvenori, applied in the field of extraction and detection of mogroside, can solve the problems of not extracting mogroside IV, and achieve the effect of novel combination technology

CN106432393AInactive Publication Date: 2017-02-22GUILIN UNIV OF ELECTRONIC TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

Disclosed is a method of extracting and detecting mogrosides IV and V from momordica grosvenori of 55 days in fruiting age. The method includes: adopting a 60% alcohol water solution added with ultrasonic assisted extraction, using Ca(OH)2 to flocculate, filter, concentrate and recycle alcohol; adopting D101 macroporous resin for separation and purification: when pH is 9.0, using flowing speed of twice column volume per hour for column chromatography, sequentially using pure water and 20% alcohol for cleaning, and using 60% alcohol for elution; collecting 60% alcohol eluant, depressurizing for concentration, and drying; using HPLC (high performance liquid chromatography) for detection, wherein chromatographic column conditions include that a chromatographic column is C18 column, a ratio of acetonitrile to water solution is 21:79, detection wavelength is 203nm, flowing speed is 1 mL / min, column temperature is 25 DEG C, feeding amount is 10uL, and column chromatography is performed for 30 min. During detection, absorbing peaks of the mogrosides IV and V are obvious, content of the mogroside IV is greater than 5.0% while that of the mogroside V is greater than 15.0%, synchronous extraction and detection of the mogrosides IV and V are realized, and the method is of industrial significance.
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Description

technical field

[0001] The invention relates to the field of plant extraction, in particular to a method for extracting and detecting mogroside from Luo Han Guo. Background technique

[0002] The cucurbitane-type triterpene glycosides in Luo Han Guo are the main part of sweet substances, mainly mogroside IV (mogroside IV) and mogroside V (mogroside V). The molecular structure of mogroside IV and mogroside V differs by one glucose group , which are close in nature and difficult to separate. The invention patent with the publication number CN104069152 A discloses a preparation method of an extract with high content of mogroside IV, Luo Han Guo → Grinding → Extraction → Filtration → Filtrate → Membrane separation → Adsorption separation → Elution 1 → Ethanol recovery 1 → Resin Decolorization→drying→crude mogroside→water dilution→macroporous resin adsorption→elution 2→ethanol recovery 2→drying under reduced pressure→ Luo Han Guo extract. Liu Jinlei, Li Dianpeng, Huang Yonglin,...

Examples

Embodiment 1

[0041] Synchronous extraction method of high-content mogroside IV and mogroside V extracts:

[0042] 1. Take 2 fresh grosvenor fruits with a fruit age of 55 days, wipe them clean, weigh them and make a record, mash them with a masher to obtain grosvenor pulp; add 60% ethanol aqueous solution at a solid-to-liquid ratio of 1:2, and place in an ultrasonic instrument middle. Ultrasonic conditions: ultrasonic instrument frequency 53HZ, power 100W, temperature 50°C, take out after ultrasonic for 30min, filter with gauze after cooling, take the filtrate, extract the filter residue three times in the same way, combine the filtrate three times, and set aside.

[0043] Add 10% Ca(OH) of 15% by weight of Luo Han Guo pulp 2 Suspended liquid, and stirring, at this time, it will be found that the Luo Han Guo extract obviously has the phenomenon of macromolecular substance aggregation. After the temperature drops to room temperature, put the beaker containing the Luo Han Guo solution into t...