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A kind of preparation method of extract with high content of mogroside IV

A technology of mogroside and Luo Han Guo, which is applied in the field of preparation of Luo Han Guo extract, can solve the problem of not mentioning the content of mogroside IV, and achieve the effect of novel combination technology and high content

Active Publication Date: 2017-09-19
桂林三棱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese invention patent application publication number is CN101007042A, which discloses a decolorization and debittering method for the preparation of an extract containing mogrosides, but does not mention the content of mogroside IV

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 100kg of fresh grosvenor fruit with a fruit age of 50 days, add 200kg of water, grind the grosvenor fruit and water together with a grinder; transfer it to a 1-ton ultrasonic extraction tank, and then add 600kg of 20% ethanol solution for ultrasonic reflux extraction 1 hour; filter after the extraction to obtain the filtrate; add 600kg of 20% ethanol solution to the Luo Han Guo filter residue, repeat the extraction 3 times, combine the resulting filtrates to obtain the extract 1; filter the extract 1 with a 0.45 μm ceramic membrane, The microfiltrate is obtained, and the microfiltrate is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 1000, and the ultrafiltration retentate is collected to obtain a concentrated solution 1; the concentrated solution 1 is injected into an AB-8 adsorption resin column, and the column effluent is discarded; After completion, elute with 20% ethanol solution of 2 times of column bed volume until the colum...

Embodiment 2

[0027] Take 100kg of fresh grosvenor fruit with a fruit age of 40 days, add 200kg of water, grind the grosvenor fruit and water together with a grinder; transfer it to a 1-ton ultrasonic extraction tank, and then add 600kg of 20% ethanol solution for ultrasonic reflux extraction 2 hours; filter after the extraction to obtain the filtrate; add 600kg of 20% ethanol solution to the Luo Han Guo filter residue, repeat the extraction twice, and combine the obtained filtrates to obtain the extract 1; filter the extract 1 with a 0.22µm ceramic membrane , to obtain a microfiltrate, the microfiltrate is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 1000, and the ultrafiltration retentate is collected to obtain a concentrated solution 1; the concentrated solution 1 is injected into the AB-8 adsorption resin column, and the column effluent is discarded; After the adsorption is completed, elute with 20% ethanol solution of 2 times the volume of the column...

Embodiment 3

[0029] Take 100kg of fresh grosvenor fruit with a fruit age of 30 days, add 200kg of water, grind the grosvenor fruit and water together with a grinder; transfer it to a 1-ton ultrasonic extraction tank, and then add 600kg of 20% ethanol solution for ultrasonic reflux extraction 1 hour; filter after the extraction to obtain the filtrate; add 600kg of 20% ethanol solution to the Luo Han Guo filter residue, repeat the extraction twice, and combine the obtained filtrate to obtain the extract 1; filter the extract 1 with a 0.45µm ceramic membrane , to obtain a microfiltrate, the microfiltrate is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 1000, and the ultrafiltration retentate is collected to obtain a concentrated solution 1; the concentrated solution 1 is injected into the AB-8 adsorption resin column, and the column effluent is discarded; After the adsorption is completed, elute with 20% ethanol solution of 2 times the volume of the column b...

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Abstract

A method for preparing an extract with high content of mogroside IV, using fresh Luo Han Guo as raw material, adopting the following process steps: Luo Han Guo→grinding→extracting→filtering→filtrate→membrane separation→adsorption separation→elution 1→ethanol recovery 1→resin Decolorization→drying→crude mogroside→water dilution→macroporous resin adsorption→elution 2→ethanol recovery 2→drying under reduced pressure→Mogroside extract. Using fresh grosvenor fruit with a fruit age of 30-50 days, through grinding, extraction, filtration, membrane separation, adsorption separation, elution, ethanol recovery, resin decolorization, and drying, the crude mogroside is obtained; the crude mogroside is further processed by large The pore resin is adsorbed, and the eluting time is 100 minutes with 70% ethanol solution, and the selective elution is carried out, the ethanol is recovered, and dried to obtain the Luo Han Guo extract with the mogroside IV content of 15%-75%.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to a preparation method of Luo Han Guo extract. Background technique [0002] Luo Han Guo is a perennial herbaceous vine of the family Cucurbitaceae. It is the first batch of precious Chinese medicinal materials for both medicine and food announced by the Ministry of Health. It is known as the "fairy fruit". Luo Han Guo has high nutritional value and contains mogrosides, among which mogroside V is the main component of dried Luo Han Guo, accounting for about 0.5% of the dried fruit. The content of mogroside IV is very small, and the content is higher when the fruit is 30-50 days old, and it gradually disappears during the ripening process. There is basically no mogroside IV in the mature fruit. In addition, it contains glucose, fructose, polysaccharide, protein, amino acid, flavonoid glycosides, vitamin C and various mineral elements. Dryness of the lungs, cough, whooping cough, su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/42A61P1/16A61P31/14A61K131/00
Inventor 周治德谢冬养李桂银张连香胡丹龙周晓青
Owner 桂林三棱生物科技有限公司
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