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Low-molecular-weight heparin molecular weight and molecular weight distribution detection method

A technology of molecular weight distribution and low molecular weight heparin, which is applied in the field of molecular weight and molecular weight distribution detection of anticoagulant drug low molecular weight heparin, can solve the problems of accuracy and sensitivity to be improved, complex operation, low resolution, etc., and save samples The effect of high sample volume, high resolution and high sensitivity

Active Publication Date: 2017-02-22
SHENZHEN TECHDOW PHARM CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0017] The above method is complex in operation, long in cycle, low in efficiency, low in resolution while accuracy and sensitivity need to be improved

Method used

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  • Low-molecular-weight heparin molecular weight and molecular weight distribution detection method
  • Low-molecular-weight heparin molecular weight and molecular weight distribution detection method
  • Low-molecular-weight heparin molecular weight and molecular weight distribution detection method

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Embodiment 1

[0063] Step 1, preparation of low-molecular-weight heparin sodium standard solution: Weigh about 20 mg of low-molecular-weight heparin sodium standard, add mobile phase to dissolve into a 10 mg / ml standard solution, filter it into a sample bottle with a disposable 0.22um filter membrane, and paste it logo. Seal the standard solution with parafilm and freeze it for storage. Take out and equilibrate to room temperature before use and mix well before use.

[0064] Step 2, preparation of low molecular weight heparin sodium molecular weight calibration standard solution: weigh 10 mg of low molecular weight heparin sodium molecular weight calibration standard, add filtered mobile phase to dissolve into a 10 mg / ml calibration solution. Distribute the calibration solution into several inner tubes, each filled with about 150ul. Seal the aliquoted solution with parafilm and store in a freezer. Take out and equilibrate to room temperature before use and mix well before use.

[0065] ...

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Abstract

The invention relates to a low-molecular-weight heparin molecular weight and molecular weight distribution detection method. High performance liquid chromatography is used, the method includes the following steps that firstly, a low-molecular-weight heparin sodium standard substance solution is prepared; secondly, a low-molecular-weight heparin sodium molecular weight calibration standard solution is prepared; thirdly, a test sample solution is prepared; fourthly, a 0.01% sodium azide solution is prepared; fifthly, the 0.01% sodium azide solution, the low-molecular-weight heparin sodium standard substance solution, the low-molecular-weight heparin sodium molecular weight calibration standard solution and the test sample solution are injected into a high performance liquid chromatograph respectively to obtain a chromatogram, the molecular weight of a test sample is calculated and a molecular weight distribution result is obtained according to the chromatogram.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a molecular weight and molecular weight distribution detection method of low molecular weight heparin, an anticoagulant drug. Background technique [0002] Low molecular weight heparin is a general term for a class of lower molecular weight heparins prepared by depolymerization of unfractionated heparin. The average molecular weight is 4000-6000, which can be divided into different commercial preparations according to the molecular weight, chain end structure and compound binding salts. Currently, dalteparin sodium, enoxaparin sodium and nadroparin calcium are mainly used in the Chinese market. It is a colorless or light yellow clear liquid. [0003] Low molecular weight heparin has the following drug effects [0004] (1) Prevention of deep vein thrombosis and pulmonary embolism. [0005] (2) Treatment of acute deep venous thrombosis that has formed. [0006] (3) Prevent thrombus or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 李建科周洋石瑛王景文胡默青
Owner SHENZHEN TECHDOW PHARM CO LTD
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