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Function and application of dual specificity phosphatase 14 in the treatment of non-alcoholic fatty liver disease and type Ⅱ diabetes

A fatty liver, right and wrong technology, used in gene therapy, metabolic diseases, pharmaceutical formulations, etc.

Active Publication Date: 2019-12-10
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the therapeutic effect of controlling hyperlipidemia in T2DM patients with NASH remains to be explored, the treatment of NAFLD mainly includes active control of diabetes and cardiovascular risk factors

Method used

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  • Function and application of dual specificity phosphatase 14 in the treatment of non-alcoholic fatty liver disease and type Ⅱ diabetes
  • Function and application of dual specificity phosphatase 14 in the treatment of non-alcoholic fatty liver disease and type Ⅱ diabetes
  • Function and application of dual specificity phosphatase 14 in the treatment of non-alcoholic fatty liver disease and type Ⅱ diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] [Example 1] Fatty liver in mice and type Ⅱ diabetes model (diet induced obesity, DIO) were obtained

[0058] (1) Grouping of experimental animals: 8-week-old, male, WT mice and DUSP14-KO mice were selected and given two special diets, D12942 high-fat diet (High fat diet, HFD) and D12450B low-fat diet (Normal chow , NC) feeding, that is, WT NC group, KO NC group, WT HFD group, KO HFD group, a total of 4 groups.

[0059] (2) The model induces the operation process through high-fat feed:

[0060] WT and KO mice were used to establish DIO models, and phenotype correlation analysis was performed to clarify the role of DUSP14 gene in fatty liver and type Ⅱ diabetes. 8-week-old, male, WT mice and DUSP14-DUSP14 mice were selected and fed with two special diets, D12942 high-fat diet (Highfat diet, HFD) and D12450B low-fat diet (Normal chow, NC), respectively, namely WT NC group, KO NC group, WT HFD group, KO HFD group, a total of 4 groups. The food intake of the mice was reco...

Embodiment 2

[0061] [Example 2] Determination of fasting blood glucose level and serum insulin level in mice

[0062] (1) Detection of fasting blood glucose level in mice

[0063] All the mice to be tested were fasted from 8:00 am to 2:00 pm (without water), that is, the experimental operation was started after 6 hours of fasting.

[0064]① Blood glucose meter preparation: Check the battery of the blood glucose meter (Johnson & Johnson, ONETOUCH, USA), press the switch on the right, put the test strip into the left slot correctly, the screen will display the number corresponding to the code of the blood glucose test strip, and then display the pattern of dripping blood, It prompts the blood glucose meter to enter the standby state.

[0065] ②Fix the mouse: hold the tail of the mouse in the right hand, hold a towel in the left hand, fold the towel in half, pinch the folded part of the towel with the thumb and forefinger, wrap the head and body of the mouse in the towel in the palm, place t...

Embodiment 3

[0088] [Example 3] Glucose tolerance test (intraperitoneal glucose tolerance test, IPGTT)

[0089] In the 12th week of the experiment, the intraperitoneal glucose injection test (IPGTT) was performed to evaluate the glucose tolerance of the mice.

[0090] (1) Before measuring blood glucose, measure the fasting body weight of the mice, and calculate the injection volume of glucose based on 10 μL / g.

[0091] (2) First detect the fasting blood glucose at 0 minutes before the glucose injection, and inject the glucose solution intraperitoneally quickly after the detection is completed.

[0092] (3) Operation method of intraperitoneal injection: ①Fix the mouse; grab the mouse, grab the tail of the mouse with the little finger and ring finger of the left hand, and grab the neck of the mouse with the other three fingers, so that the head of the mouse is downward, and the The abdomen of the mouse is fully exposed. ②Needle positioning and injection: insert the needle from the side of ...

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Abstract

The invention discloses a function and use of a DUSP14 gene in treatment on fatty liver and diabetes. DUSP14 knockout mice and wild type C57 mice are as experimental subjects, an obese mouse model induced by high fat diet is used, a test result shows that compared with the wild type C57 mice, DUSP14-KO mice are obese, have fasting blood glucose levels higher than those of the control group WT mice and have liver functions significantly worse than those of the WT mice. An intraperitoneal injection-based glucose tolerance test proves that DUSP14-KO mouse tolerance to glucose is significantly reduced. According to liver weight, a liver / body weight ratio and a lipid pathological staining result, it is shown that the DUSP14-KO mice induced by high fat diet suffer severe fatty liver and fat accumulation is significantly increased. Therefore, DUSP14 can be used as a drug target for screening and treating fatty liver and / or type II diabetes. The accelerator can be used to prepare drugs for treating fatty liver and / or type II diabetes.

Description

technical field [0001] The invention belongs to the field of gene function and application, in particular to a kind of dual-specificity phosphatase 14 (Dual-specificity phosphatase 14, DUSP14) as a target gene in the preparation of prevention, alleviation and / or treatment of fatty liver and / or type II diabetes application in medicine. Background technique [0002] Diabetes is caused by various factors such as genetic factors, immune dysfunction, microbial infection, and mental factors. The body's islet function is reduced, insulin resistance, and finally leads to a series of metabolic disorder syndromes such as sugar, protein, fat, water, and electrolytes. Type 2 diabetes mellitus (T2DM), also known as non-insulin-dependent diabetes mellitus, is a metabolic syndrome characterized by hyperglycemia [1] Diabetes, accounting for more than 90% of the total number of people with diabetes, has become the third largest disease affecting human health after cancer and cardiovascular ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P3/10A61P1/16
CPCA61K48/005
Inventor 李红良黄赞
Owner WUHAN UNIV
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