Method for tissue culture of ice vegetable seeds and stem segments

A technology of tissue culture and frozen vegetables, which is applied in the field of technical regeneration through tissue culture, can solve the problems of difficult control of the salt content of the culture medium, poor taste of ice vegetables, and insufficient robustness of the plants, and achieves simple methods, significant effects, and high yields. high effect

Inactive Publication Date: 2019-01-15
TIANJIN AGRICULTURE COLLEGE
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in the large-scale production of ice vegetables, the salt content in the culture medium is not easy to control, and the conventional seed and stem section tissue culture method is used for cultivation, the effect is not ideal, or the obtained plants are not strong enough, or the growth is not good. , the taste of the cultivated ice vegetables is not good

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for tissue culture of ice vegetable seeds and stem segments
  • Method for tissue culture of ice vegetable seeds and stem segments
  • Method for tissue culture of ice vegetable seeds and stem segments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In the method for tissue culture of iced vegetable seeds of this embodiment, the method includes the following steps:

[0026] 1. Medium preparation: adjust the concentration of sucrose and agar in MS medium, then add NaCl to MS medium and adjust the pH to 5.8 to obtain iced vegetable medium;

[0027] 2. Seed treatment: Put the iced vegetable seeds into a triangular flask, add 40mL of water and 2 drops of Tween 80 (surfactant), shake well (5min); then rinse the iced vegetable seeds 5 times with water and drain the water; In the ultra-clean workbench, soak and shake with 75% alcohol for 30s, and rinse once with sterile water; after soaking and shaking with 10% sodium hypochlorite solution for 15 minutes, rinse with sterile water for 4-5 times; take out the iced vegetable seeds and put them in In a petri dish with sterile filter paper, absorb the water;

[0028] 3. Inoculation and cultivation: Take the iced vegetable seeds obtained in step 2 and place them on the medium prepare...

Embodiment 2

[0031] In the method for tissue culture of iced vegetable seeds of this embodiment, the method includes the following steps:

[0032] 1. Medium preparation: adjust the concentration of sucrose and agar in MS medium, then add NaCl to MS medium and adjust the pH to 5.8 to obtain iced vegetable medium;

[0033] 2. Seed treatment: Put the iced vegetable seeds into a triangular flask, add 40mL of water and 2 drops of Tween 80 (surfactant), shake well (5min); then rinse the iced vegetable seeds 5 times with water and drain the water; In the ultra-clean workbench, soak and shake with 75% alcohol for 30s, and rinse once with sterile water; after soaking and shaking with 10% sodium hypochlorite solution for 15 minutes, rinse with sterile water for 4-5 times; take out the iced vegetable seeds and put them in In a petri dish with sterile filter paper, absorb the water;

[0034] 3. Inoculation and cultivation: Take the iced vegetable seeds obtained in step two and place them on the medium prepa...

Embodiment 3

[0037] In the method for tissue culture of iced vegetable seeds of this embodiment, the method includes the following steps:

[0038] 1. Medium preparation: adjust the concentration of sucrose and agar in MS medium, then add NaCl to MS medium and adjust the pH to 5.8 to obtain iced vegetable medium;

[0039] 2. Seed treatment: Put the iced vegetable seeds into a triangular flask, add 40mL of water and 2 drops of Tween 80 (surfactant), shake well (5min); then rinse the iced vegetable seeds 5 times with water and drain the water; In the ultra-clean workbench, soak and shake with 75% alcohol for 30s, and rinse once with sterile water; after soaking and shaking with 10% sodium hypochlorite solution for 15 minutes, rinse with sterile water for 4-5 times; take out the iced vegetable seeds and put them in In a petri dish with sterile filter paper, absorb the water;

[0040] 3. Inoculation and cultivation: Take the iced vegetable seeds obtained in step 2 and place them on the culture medium...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for culturing seed tissues and stem tissues of mesembryanthemum crystallinum, relates to a technical regeneration method based on tissue culture, and particularly relates to a tissue culture based technical regeneration method for the mesembryanthemum crystallinum. According to the method for culturing the seed tissues of the mesembryanthemum crystallinum, sprouting ratio is high, and growth tread is good; plant height and fresh weight of the mesembryanthemum crystallinum prepared according to the method are remarkably higher than mesembryanthemum crystallinum prepared according to conventional methods. The method for culturing the seed tissues of the mesembryanthemum crystallinum includes culture medium preparation, seed treatment, inoculation and culture. The method for culturing the stem tissues of the mesembryanthemum crystallinum includes culture medium preparation, inoculation material preparation, inoculation and culture. The method has the advantages that through addition of saline solutions into culture media, mesembryanthemum crystallinum tissue culture seedlings growing well under high saltness (0.9%) conditions are obtained and are applied to salt-resisting production. The method is applied to the field of mesembryanthemum crystallinum planting.

Description

Technical field [0001] The present invention relates to a method for regeneration through tissue culture technology, and more particularly to a method for regeneration through tissue culture technology for ice vegetable. Background technique [0002] With the development of biological sciences and the increasing degree of salinization of farmland and woodland in the world, the introduction of salt-tolerant varieties and the improvement of plant salt-tolerant research have received more and more attention. The iced vegetable juice contains a certain amount of salt, which can be improved in saline-alkali land. , Food and other aspects have high application value. [0003] In the current large-scale production of iced vegetable, the salt content in the culture medium is not easy to control, and the conventional seed and stem tissue culture method is used for cultivation, the effect is not satisfactory, or the obtained plant is not strong enough, or the growth is not good , The cultiv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 荣海燕潘淑芬王坤孙秀润
Owner TIANJIN AGRICULTURE COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap