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A pretreatment method and application for carbon stable isotope detection of plant samples

A carbon stable isotope, plant technology, applied in the fields of botanical equipment and methods, applications, horticultural methods, etc., can solve the problems of long-term water stress treatment of perennial plants, and achieve accurate and reliable detection data and results, good uniformity, and improved The effect of efficiency

Inactive Publication Date: 2019-07-05
INST OF DRY LAND FARMING SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a pretreatment method for plant samples used for carbon stable isotope detection, which solves the problem that polyethylene glycol solution stress treatment is not suitable for long-term water stress treatment of perennial plants in existing plant sample pretreatment methods

Method used

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  • A pretreatment method and application for carbon stable isotope detection of plant samples

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for pretreatment of plant samples for carbon stable isotope detection, specifically comprising the following steps:

[0040] Step 1: Air-dry the perlite and quartz sand respectively, air-dry them naturally, then mix the perlite and quartz sand in a volume ratio of 1:2 to obtain a perlite-quartz sand mixture, then place it in a container, add nutrient solution and soak for 12 hours , and then drain excess water to obtain a perlite-quartz sand matrix.

[0041] Wherein, the nutrient solution contains the following components: 0.6g / L of KNO 3 , 0.9g / L Ca(NO 3 ) 2, 0.23g / L of NH 4 h 2 PO 4 , 0.12g / L of MgSO 4 , 3.7mg / L KCl, 0.6mg / L H 3 BO 3 , 0.3mg / L of MnSO 4 , 0.32mg / L ZnSO 4 , 0.13mg / L of CuSO 4 , 0.08mg / L of (NH4) 6 Mo 7 o 24 , 18.8mg / L of Fe-EDTA, the solvent is water.

[0042] Step 2, according to the size of the plant seedlings to be detected, select a cultivation container, put the perlite-quartz sand matrix into the container, and plant the pl...

Embodiment 2

[0048] A method for pretreatment of plant samples for carbon stable isotope detection, specifically comprising the following steps:

[0049] Step 1: Air-dry the perlite and quartz sand respectively, air-dry them naturally, then mix the perlite and quartz sand at a volume ratio of 1:9 to obtain a perlite-quartz sand mixture, then place it in a container, add nutrient solution and soak for 24 hours , and then drain excess water to obtain a perlite-quartz sand matrix.

[0050] Wherein, the nutrient solution contains the following components: 0.65g / L of KNO 3 , 0.95g / L of Ca(NO 3 ) 2 , 0.20g / L NH 4 h 2 PO 4 , 0.10g / L of MgSO 4 , 3.6mg / L of KCl, 0.62mg / L of H 3 BO 3 , 0.29mg / L of MnSO 4 , 0.31mg / L of ZnSO 4 , 0.11mg / L of CuSO 4 , 0.079mg / L of (NH4) 6 Mo 7 o 24 , 18.6mg / L of Fe-EDTA, the solvent is water.

[0051] Step 2, according to the size of the plant seedlings to be detected, select a cultivation container, put the perlite-quartz sand matrix into the container, ...

Embodiment 3

[0057] A method for pretreatment of plant samples for carbon stable isotope detection, specifically comprising the following steps:

[0058] Step 1: Air-dry the perlite and quartz sand respectively, air-dry them naturally, then mix the perlite and quartz sand in a volume ratio of 1:1 to obtain a perlite-quartz sand mixture, then place it in a container, add nutrient solution and soak for 18 hours , and then drained to obtain a perlite-quartz sand matrix.

[0059] Wherein, the nutrient solution contains the following components: 0.63g / L of KNO 3 , 0.93g / L Ca(NO 3 ) 2 , 0.25g / L NH 4 h 2 PO 4 , 0.15g / L of MgSO 4 , 3.8mg / L of KCl, 0.6mg / L of H 3 BO 3 , 0.29mg / L of MnSO 4 , 0.33mg / L of ZnSO 4 , 0.11mg / L of CuSO 4 , 0.08mg / L of (NH4) 6 Mo 7 o 24 , 18.7mg / L of Fe-EDTA, the solvent is water.

[0060] Step 2, according to the size of the plant seedlings to be detected, select a cultivation container, put the perlite-quartz sand matrix into the container, and plant the pl...

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Abstract

The invention discloses a pretreatment method for carbon stable isotope detection of a plant sample. The pretreatment method comprises the following steps: mixing perlite and quartz sand according to the volume proportion of 1:1-9, air-drying, adding nutrient solution and soaking, and then draining the excessive moisture, and obtaining a perlite and quartz sand matrix; planting plant seedlings to be detected; performing the transplanting seedling cultivation and the water stress cultivation; taking healthy and whole leaves of the plants, washing and removing the excessive moisture on the surface, sterilizing for 15 minutes in 105 DEG C, and then drying in the constant temperature of 65 DEG C until the weight is constant, obtaining the dried leaves; and taking the dried leaves, grinding to be fine powder, sieving by 100 meshes of a sieve, and obtaining a sample for the carbon stable isotope detection. The method is suitable for the long-time water stress treatment of the perennial plant, the test uniformity is better, and the impact factor in the test is single relatively. The test interference factor is easily eliminated, the controllability is better, the more ideal water controlling effect can be realized, and the diction data and result are more accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of stable isotope analysis, and in particular relates to a pretreatment method and application for carbon stable isotope detection of plant samples. Background technique [0002] In the dwarfing and dense planting cultivation of pears, the comprehensive cultivation traits of pears' dwarfing rootstock and root stock combination (combination of rootstock and variety) is an important research content, and wherein, water use efficiency and biological water saving are another item. important physiological indicators. At present, in the study of pear cultivation physiology, the most widely used method is to measure instantaneous water use efficiency by using a portable photosynthetic system, but the measurement results cannot reflect the overall situation of long-term water use efficiency (WUE) in the growth cycle of pears, and the screening cycle Longer, the accuracy of the results is not good enough, and it has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G7/06
CPCA01G7/06A01G31/00
Inventor 邵嘉鸣张祖维姜晓东李新凤张述义顾蓉张小宁刘玲玲
Owner INST OF DRY LAND FARMING SHANXI ACAD OF AGRI SCI
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