Chronic kidney disease marker suPAR detection kit and preparation method thereof

A chronic kidney disease and detection kit technology, applied in the field of medical products, can solve the problems of complex operation and lack of research on chronic kidney disease, and achieve the effects of simple operation, convenient measurement, high accuracy and specificity

Inactive Publication Date: 2017-04-05
上海凯璟生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Danish scholars established uPAR or suPAR ELISA detection kits for the diagnosis or prognosis of Streptococcus pneumoniae and Mycobacterium tuberculosis infections [12] , the method is complicated to operate, and the article does not reflect the relevant research on chronic kidney disease

Method used

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  • Chronic kidney disease marker suPAR detection kit and preparation method thereof
  • Chronic kidney disease marker suPAR detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preparation of dry immunofluorescence kit for chronic kidney disease suPAR of the present invention

[0017] The kit of the present invention comprises a PVC base plate, a fluorescent binding pad, a nitrocellulose membrane, a sample pad and a water-absorbing pad; the sample pad, the nitrocellulose membrane, the fluorescent binding pad, and the water-absorbing pad are fixed on the PVC base plate in a horizontal direction.

[0018] 1. Preparation of nitrocellulose membrane (NC membrane)-PVC bottom plate

[0019] 1. Scribing film: Cut the nitrocellulose into 25mm×300mm and paste it on the PVC bottom plate. Dilute the rabbit anti-chicken IgY with PBS to a concentration of 1 mg / ml as the C-line coating solution; dilute the mouse anti-human SUPAR monoclonal antibody 2 with PBS to a concentration of 1 mg / ml as the T-line. Using a film sprayer, streak the T-line coating solution and C-line coating solution on the nitrocellulose membrane to obtain the test line and qu...

Embodiment 2

[0028] Embodiment 2 The usage method of kit of the present invention

[0029] 1. Use a pipette to pipette 50ul serum, plasma, and cerebrospinal fluid samples and add them to the sample pad of the test strip;

[0030] 2. Stand at room temperature for 10 minutes;

[0031] 3. At the end of 10 minutes, put the test strip into the immunofluorescence quantitative analyzer to read the data;

[0032] 4. The immunofluorescence quantitative analyzer measures and analyzes the optical signal, and quantitatively obtains the concentration of the tested substance;

[0033] 5. Judgment of negative and positive according to the reference value.

Embodiment 3

[0034] Embodiment 3 Comparison between the kit of the present invention and the ELISA kit

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Abstract

The invention relates to a chronic kidney disease marker suPAR detection kit and a preparation method thereof. The kit includes a PVC base board, a fluorescent combining pad, a nitrocellulose membrane, a sample pad, and a water absorption pad. The sample pad, the nitrocellulose membrane, the fluorescent combining pad and the water absorption pad are fixed on the PVC base board in the horizontal direction. A coated rat-anti-human suPAR monoclonal antibody 1-nano fluorescent microsphere compound and a chicken IgY-nano fluorescent microsphere compound are formed on the fluorescent combining pad; and a detection line, which is formed by an rat-anti-human suPAR monoclonal antibody 2, and a quality control line, which is fomed by a rabbit-anti-chicken IgY antibody, are formed on the nitrocellulose membrane. Through nano fluorescent microspheres for detection of suPAR, the kit has low background value, short detection time, high specificity and sensitivity and accurate detection result. The kit provides a more reliable basis for early stage clinical diagnosis and general survey of the chronic kidney disease.

Description

technical field [0001] The invention relates to a chronic kidney disease marker suPAR detection kit and a preparation method, and the invention belongs to the field of medical products. Background technique [0002] Kidney disease (KD) is chronic kidney structure and dysfunction caused by various reasons. There are many kinds of kidney diseases. Patients with blood pressure and systemic lupus erythematosus are also often complicated by kidney disease. In the past 10 years, the research on chronic kidney disease (CKD) has become a hot spot that has attracted wide attention internationally. National surveys in developed countries such as the United States, Germany, and Norway show that CKD is a common chronic disease, and the prevalence of CKD in the adult population is 10.2% to 13.0%. [1-5] , while the adult prevalence rate in China is 10.8% [6-7] . Chronic kidney disease is characterized by high prevalence, high mortality, and low awareness, and has become the most commo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/705G01N2800/347
Inventor 罗朝领董敏卢荣春
Owner 上海凯璟生物科技股份有限公司
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