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A kind of manganese ion colorimetric detection kit and its detection method

A detection kit and manganese ion technology, applied in the field of environmental and biological analysis, can solve the problems of high price, complicated detection methods, inability to realize on-site detection and technical promotion, etc., and achieve the effects of easy observation, good selectivity and fast speed.

Active Publication Date: 2020-02-21
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies require expensive large-scale instruments, or the detection methods are too complicated to realize on-site detection and technology promotion.

Method used

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  • A kind of manganese ion colorimetric detection kit and its detection method
  • A kind of manganese ion colorimetric detection kit and its detection method
  • A kind of manganese ion colorimetric detection kit and its detection method

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Embodiment Construction

[0032] Through the following examples, the present invention will be further specifically described. However, the present invention is by no means limited thereto.

[0033] The kit of the present invention adopts weak acid acetic acid with a pH of 3.6-5.8 and a concentration of 0.01-1M, so that the manganese-catalyzed potassium periodate oxidation reaction is under weakly acidic conditions, and CTAB-modified gold nanorods, wherein CTAB is used as a surface The active agent has the effect of sensitization and nitrilotriacetic acid, which promotes the catalytic effect. During the reaction process, the gold nanorods are blue under normal conditions. After being oxidized and etched by potassium periodate, their shape changes from rod shape to spherical shape. From blue to red, this change has a certain linear relationship with the concentration of manganese ions added as a catalyst, and the detection of manganese ions is performed according to this linear relationship.

[0034] M...

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PUM

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Abstract

The invention belongs to the technical field of environmental and biological analysis, and more specifically relates to a manganese ion colorimetry detection kit, and a detection method thereof. The manganese ion colorimetry detection kit is composed of acetic acid-sodium acetate buffer solution, a potassium periodate solution, a nitrilotriacetic acid solution, a gold nanorod solution containing cetyl trimethyl ammonium bromide (CTAB), a standard colourimetric card C, and a colorimetric tube; in the gold nanorod solution containing cetyl trimethyl ammonium bromide (CTAB), the concentration of nano rod is controlled to be 0.5 to 10nM, and the concentration of CTAB is controlled to be 0.01 to 100mM. The detection method is high in sensitivity and selectivity, and is simple in operation; naked-eye observation can be realized easily; and detection of manganese ions in environment samples can be realized under systematic experiment condition debugging.

Description

technical field [0001] The invention relates to the technical field of environmental and biological analysis, in particular to a manganese ion colorimetric detection kit and a detection method thereof. Background technique [0002] Manganese is one of the essential trace elements for the human body. It plays an important role in the composition of many enzymes and is also an essential component of normal bone structure. Manganese deficiency can cause neurasthenic syndrome, and even lead to a decrease in insulin synthesis and secretion; while manganese excess may cause poisoning. Occupational manganese poisoning is caused by long-term inhalation of manganese fume and manganese dust with high manganese content. Especially workers in ferromanganese smelting, welding rod manufacturing and welding operations, manganese ore mining, crushing or dry battery production, long-term exposure to manganese can cause neurological symptoms such as Parkinson's syndrome or Wilson's disease. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/33G01N21/78
Inventor 陈兆鹏张耀文陈令新张志阳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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