Applications of abdominal cavity cells in treatment of immune diseases

An immune disease and autoimmune technology, applied in allergic diseases, metabolic diseases, bone diseases, etc., can solve problems such as difficult treatment, and achieve the effect of obvious curative effect, easy access, and good application prospects.

Inactive Publication Date: 2017-04-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regulatory T cells and mesenchymal stem cells both have immunosuppressive effects in the treatment of autoimmune diseases, but it is very difficult to obtain enough regulatory T cells and mesenchymal stem cells from tissues for treatment

Method used

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  • Applications of abdominal cavity cells in treatment of immune diseases
  • Applications of abdominal cavity cells in treatment of immune diseases
  • Applications of abdominal cavity cells in treatment of immune diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1. Obtaining peritoneal cells and using them for treating inflammatory bowel disease.

[0063] 1. Acquisition of peritoneal cells and their component cells

[0064] 1. The mice were killed by cervical dislocation, soaked in 75% alcohol for 1-2 minutes, moved into the ultra-clean table, fixed on the dissecting board in the supine position, and disinfected the abdominal skin with alcohol.

[0065] 2. Use a 10ml syringe fitted with a 25g needle to draw 8mL of RPMI-1640 medium into the abdominal cavity, rub the abdomen lightly, then cut a small opening in the neck with ophthalmic scissors, and cut the abdominal skin along the midline of the abdomen from the small opening to expose the abdominal muscles layer.

[0066] 3. Under sterile conditions, draw the cell suspension with a 10ml syringe and transfer it into a centrifuge tube.

[0067] 4. After counting, centrifuge at 1200rpm for 5min, resuspend the cells and dilute to the target concentration in RPMI-1640 c...

Embodiment 2

[0083] Embodiment 2, treating rheumatoid arthritis, myocarditis and psoriasis with peritoneal cells

[0084] 1. Non-specific immunosuppressive effect of peritoneal cells

[0085] The experiment was divided into three groups: (1) healthy mouse control group (control); (2) OVA immunized group (OVA); (3) OVA immunized peritoneal cell therapy group (OVA+PC). The details are as follows:

[0086] (1) Healthy mouse control group

[0087] No special treatment was done from the beginning to the end of the experiment.

[0088] (2) OVA immune group and OVA immune peritoneal cell therapy group

[0089] 1. Experimental method: On the 0th day and the 14th day of the experiment, mice were subcutaneously injected with 20ug OVA. On this basis, the OVA immune group was injected with 100ul normal saline in the tail vein on the 0th day and the 14th day; the OVA immune peritoneal cell therapy group was injected with 1x10 6 / 100ul peritoneal cells.

[0090] 2. On the 10th and 21st days, the orb...

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Abstract

The present invention belongs to the field of immunotherapy, and particularly relates to applications of abdominal cavity cells in preparation of drugs for treatment of immune diseases. A technical problem to be solved by the present invention is to provide a new way for treating autoimmune diseases, organ transplantation rejection reactions and other immune diseases. The technical scheme for solving the technical problem is providing uses of abdominal cavity cells in preparation of drugs for prevention and/or treatment of immune diseases. According to the present invention, the results of a lot of tests prove that the abdominal cavity cells can provide significant treatment effects in a variety of immune diseases; and the abdominal cavity cells have advantages of easy obtaining, simple and safe use and significant treatment effect, provide the new effective selection for the treatment of immune diseases, and have good application prospects.

Description

technical field [0001] The invention belongs to the field of immunotherapy, and in particular relates to the application of peritoneal cells in the preparation of drugs for treating immune diseases. Background technique [0002] Autoimmune diseases refer to diseases caused by the body's immune response to self-antigens, resulting in damage to its own tissues. Including inflammatory bowel disease, systemic lupus erythematosus, rheumatoid arthritis, myocarditis, scleroderma, hyperthyroidism, juvenile diabetes and other autoimmune diseases and organ transplant rejection. Its pathogenesis is complex and related to many factors such as environment, genes, microorganisms and immune factors. Clinically, autoimmune diseases are difficult to treat, and the symptoms of most patients will be with them for life. The treatment of autoimmune diseases requires the generation of immune tolerance while anti-inflammation. However, current therapeutic drugs such as 5-aminosalicylic acid, gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/17A61K35/15A61P37/06A61P1/00A61P37/02A61P19/02A61P9/00A61P17/00A61P5/16A61P3/10
Inventor 魏于全刘婷
Owner SICHUAN UNIV
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