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Micro-extraction method of honeysuckle

An extraction method and technology of honeysuckle, which are applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of low selectivity of adsorbents, large amount of medicinal materials and adsorbents, and large consumption of organic solvents, so as to improve environmental protection benefits, High adsorption and extraction efficiency and the effect of reducing pollution

Inactive Publication Date: 2017-05-03
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims to solve the problems of low selectivity of adsorbents, large amount of medicinal materials and adsorbents, and large consumption of organic solvents existing in the existing matrix solid-phase dispersion extraction technology. Method for solid-phase dispersion extraction of effective components in honeysuckle medicinal material

Method used

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  • Micro-extraction method of honeysuckle
  • Micro-extraction method of honeysuckle
  • Micro-extraction method of honeysuckle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weighed 5 parts of honeysuckle herb powder, 25 mg each, and added them to 5 groups of mortars respectively, and weighed different types of cyclodextrins (α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, methyl-β-cyclodextrin, Cyclodextrin, dihydroxypropyl-β-cyclodextrin) 25mg each, added to 5 sets of mortars and ground with honeysuckle powder for 120s. Take five 1mL solid-phase extraction cartridges, add sieves to the bottom, fill the cartridges with ground powder respectively, add sieves to the top after filling, and place them on the solid-phase extraction apparatus. Take 0.5mL of methanol, inject it into the small column, and elute the small column one by one. The bottoms were collected with five 1.5mL centrifuge tubes, and then placed in a centrifuge, centrifuged at 13000rpm for 5min. The supernatant was taken, which was the extract of phenolic acid active ingredients in Flos Lonicerae, and samples were taken and analyzed by ultra-high performance liquid chromatogra...

Embodiment 2

[0043] Weigh 25 mg each of 9 honeysuckle medicinal powders and add them into 9 groups of mortars respectively, and weigh β-ring Add dextrin to 9 groups of mortars and grind with honeysuckle powder for 120s. Take nine 1mL solid-phase extraction cartridges, add sieve trays to the bottom, fill the cartridges with ground powder respectively, and add sieve trays to the top after filling. Place the filled 9 small columns on the solid phase extraction apparatus. Take 0.5mL of methanol and elute the small columns one by one. The bottoms were collected with nine 1.5mL centrifuge tubes, and then placed in a centrifuge and centrifuged at 13000rpm for 5min. The supernatant was collected as a sample, and analyzed by ultra-high performance liquid chromatography (UPLC).

[0044] The experimental results are shown in Table 2 below, and the data in Table 2 are peak areas.

[0045] Table 2.

[0046]

[0047] 1 represents neochlorogenic acid, 2 represents chlorogenic acid, 3 represents 3...

Embodiment 3

[0050] Weighed 6 parts of tangerine peel medicinal powder, 25 mg each, and added them to 6 groups of mortars, and weighed 6 parts of 75 mg β-cyclodextrin, respectively, and added them to 6 groups of mortars for different grinding times (60s, 80s, 100s, 60s, 80s, 100s, 120s, 140s, 160s). Take 6 solid-phase extraction columns of 1ml size, add sieve plates to the bottom, fill the columns with ground powder respectively, and add sieve plates to the top after filling. Place the filled 6 small columns on the solid phase extraction apparatus. Take 0.5ml of methanol, inject it into the small column, and elute the small column one by one. The bottom was collected with six 1.5ml centrifuge tubes, placed in an ionizer, and centrifuged at 13000rpm for 5min. The supernatant was collected as a sample, and analyzed by ultra-high performance liquid chromatography (UPLC).

[0051] The experimental results are shown in Table 3 below, and the data in Table 3 are peak areas.

[0052] table 3....

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Abstract

The invention discloses a method for extraction and detection of phenolic acid effective components in honeysuckle medicinal materials by using beta-cyclodextrin as an adsorbent for matrix solid-phase dispersion extraction technology. The method includes: adding honeysuckle herbal powder and a beta-cyclodextrin adsorbent into a mortar to conduct grinding, taking a solid-phase extraction small column, adding a sieve plate at the bottom, filling the small column with grinded solid, then adding a sieve plate at the top, placing the filled small column on a solid-phase extraction instrument, taking an eluant and injecting it into the small column, conducting elution on the small column, using a centrifuge tube to collect eluent at the bottom, then conducting volution and centrifugation on the eluent in the centrifuge tube, and taking supernatant liquid, i.e. a honeysuckle phenolic acid effective component extracted liquid. Compared with the traditional extraction method, the method used by the invention has more concise and faster steps, saves time and labour, greatly reduces the organic solvent dosage, has remarkable extraction effect, and can effectively extract phenolic acid components in honeysuckle.

Description

technical field [0001] The invention belongs to the field of extraction and detection of natural medicines. It relates to a micro-extraction method of medicinal plants, specifically, it involves the use of β-cyclodextrin as an adsorbent, combined with micro-matrix solid-phase dispersion extraction technology, a new method for extracting the active components of phenolic acids in honeysuckle . Background technique [0002] Honeysuckle is the dry flower bud or the first flower of Lonicera japonica Thunb. Honeysuckle has heat-clearing and toxic substances removing, the effect of evacuating wind-heat. For carbuncle swollen furunculosis, sore throat, erysipelas, pyretic toxin blood dysentery, wind-heat cold, fever due to febrile disease, etc. Chlorogenic acid (chlorogenic acid), neochlorogenic acid (neochlorogenic acid), isochlorogenic acid (isochlorogenic acid) are the main active ingredients of honeysuckle. Isochlorogenic acid is a mixture, and 3,5-dicaffeoylquinic acid (3,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/14
Inventor 曹君彭黎卿曹婉徐菁菁朱琼瑶张倩云
Owner HANGZHOU NORMAL UNIVERSITY
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