A method for homologous sperm to induce gynogenesis in Chinese sturgeon

A technology of gynogenesis and Chinese sturgeon, applied in the field of artificially induced parthenogenesis of Chinese sturgeon, to achieve the effect of easy cleaning operation

Active Publication Date: 2020-04-14
CHINESE STURGEON RES INST CHINA THREE GOR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificially induced gynogenesis has been reported in Russian sturgeon, scorpion sturgeon, flash sturgeon, Mississippi shovel sturgeon, European sturgeon, sterlet, Siberian sturgeon, spoon-nose sturgeon, and hybrid sturgeon, but there is no report on the use of Chinese sturgeon sperm to induce Report on gynogenesis of Chinese sturgeon

Method used

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  • A method for homologous sperm to induce gynogenesis in Chinese sturgeon

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Experimental program
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Effect test

Embodiment 1

[0041] (1) Inactivation of the genetic material of Chinese sturgeon sperm:

[0042] The weight range of 6 Chinese sturgeons (3 males and 3 males) was cultivated by the Chinese Sturgeon Research Institute of China Three Gorges Corporation, with a weight range of 60-80kg. Sperm motility is 80%-85%. In December 2013 and December 2014, eggs and sperm were collected for gynogenesis research.

[0043] Inactivation of genetic material in Chinese sturgeon spermatozoa by ultraviolet irradiation:

[0044]Take the above-mentioned Chinese sturgeon and add 8ml of sperm diluent, place the petri dish in ultraviolet light, and the irradiance of ultraviolet light is 335.6μw / cm 2 , the irradiation time is 7-8 minutes, the power of the ultraviolet lamp is 20W, the wavelength is 254nm, and the irradiation time gradient is 0min, 4min, 5min, 6min, 7min, 8min. excercise time.

[0045] The sperm dilution method is to dilute with diluent of equal osmotic pressure with Chinese sturgeon sperm, the v...

Embodiment 2

[0059] A method for homologous sperm to induce gynogenesis in Chinese sturgeon, said method comprising the following steps:

[0060] (1) Inactivation of the genetic material of Chinese sturgeon sperm:

[0061] Collect the Chinese sturgeon sperm, select the Chinese sturgeon sperm with an activity of more than 80% and mix it with the sperm diluent, and inactivate it with ultraviolet light in a petri dish pre-cooled at 0°C;

[0062] (2) Chinese sturgeon egg collection and insemination with Chinese sturgeon inactivated sperm:

[0063] Select mature Chinese sturgeon female fish, water temperature 18 ℃, artificially inject LHRH-A2 to induce labor, collect eggs by abdominal extrusion method when the effect time comes, use the inactivated sperm in the above step (1) for insemination, and sprinkle the sperm on Fertilize the eggs on the eggs, add water for cultivation and shake well, add water for washing, put them into a debonding bottle containing water for cultivation of talcum powd...

Embodiment 3

[0079] A method for homologous sperm to induce gynogenesis in Chinese sturgeon, said method comprising the following steps:

[0080] (1) Inactivation of the genetic material of Chinese sturgeon sperm:

[0081] Collect the Chinese sturgeon sperm, select the Chinese sturgeon sperm with an activity of more than 80% and mix it with the sperm diluent, and inactivate it with ultraviolet rays in a pre-cooled petri dish at 2°C;

[0082] (2) Chinese sturgeon egg collection and insemination with Chinese sturgeon inactivated sperm:

[0083] Select mature Chinese sturgeon female fish, water temperature 19.5 ℃, artificially inject LHRH-A2 to induce labor, collect eggs by abdominal extrusion method when the effect time comes, use the inactivated sperm in the above step (1) for insemination, and sprinkle the sperm on Fertilize the eggs on the eggs, add water for cultivation and shake well, add water for washing, put them into a debonding bottle containing water for cultivation of talcum pow...

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Abstract

The invention belongs to the field of artificial induction to parthenogenesis of Chinese sturgeons and particularly relates to a method for inducing Chinese sturgeon ova to perform gynogenesis through genetically inactivated Chinese sturgeon homologous sperms. The method comprises the steps of genetic material inactivation of Chinese sturgeon sperms, Chinese sturgeon ovum collection, insemination operation of the Chinese sturgeon sperms, chromosome doubling processing of Chinese sturgeon fertilized ova, breeding of gynogenesis inducing ova and identification of gynogenesis fry. High-homozygous-degree Chinese sturgeon fry can be produced by utilizing the method, an experiment material is provided for research on whole genome sequencing of the Chinese sturgeons, a fry source is provided for production of single-gender Chinese sturgeon fry, information is provided for disclosure of Chinese sturgeon gender determining mechanism, and technical support is provided for further research on parthenogenetic reproduction production.

Description

technical field [0001] The invention belongs to the field of artificially inducing Chinese sturgeon parthenogenesis, in particular to a method for inducing Chinese sturgeon eggs to undergo gynogenesis with genetically inactivated Chinese sturgeon homologous sperm. Background technique [0002] Chinese sturgeon is an ancient large fish unique to my country. It is large in size, fast in growth and good in meat quality, especially its large roe is a treasure among sturgeons. The Chinese sturgeon is a first-class national protected animal and was listed as a critically endangered species by the International Union for Conservation of Nature (IUCN) in 2010. Chinese sturgeon is a typical hermaphroditic fish, that is, it needs mature female fish and male fish to reproduce in the natural state. The sexual maturity of Chinese sturgeon takes a long time, males need at least 8-10 years, and females need at least 12-14 years. The sex ratio of the Chinese sturgeon is 1:1, and there are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 杜合军刘雪清陈磊舒德斌张建明肖衎胡亚成赵珣姜华郭柏福
Owner CHINESE STURGEON RES INST CHINA THREE GOR
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