Bulk drug bee wax GC standard finger-print and construction method thereof
A standard fingerprint and fingerprint technology, applied in the field of beeswax drug detection, can solve the problem of not being able to effectively monitor the quality of beeswax, and achieve the effects of good separation, good specificity and good stability
Active Publication Date: 2017-05-10
GUANGZHOU BAIYUNSHAN PHARM CO LTD
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Problems solved by technology
[0004] The 2010 edition of "Chinese Pharmacopoeia" only describes the identification of properties, and the physical and chemical identificatio
Method used
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Embodiment 1
[0030] The origins of batches 1-10 of beeswax are Lanzhou, Zhengzhou, Qingyang, Weihai, Chengdu, Yantai, Harbin, Dalian, Guangyuan and Jinan respectively.
[0031] 1. Instruments and reagents
[0032] Instruments: Shimadzu GC-2014C Gas Chromatograph, Shimadzu Corporation, Japan; BS224S Electronic Analytical Balance, Sensitivity 0.0001g, German Sartorius Corporation; Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System Version A, National Pharmacopoeia Commission.
[0033] Reagent: Chloroform, analytically pure, 500mL / bottle, batch number: 20131201, Guangzhou Chemical Reagent Factory.
[0034] 2. Chromatographic conditions for gas phase determination:
[0035] The chromatographic column is HP-5, the specification is 30m*0.25mm*0.1um (Agilent company product), the chromatographic column filler is (5%-phenyl)-methylpolysiloxane, and the detector is a hydrogen flame detector. The temperature of the instrument is 310°C, the temperature of the inje...
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Abstract
The invention discloses a bulk drug bee wax GC standard finger-print and a construction method thereof and belongs to the technical field of chemical analysis and bee wax quality detection. The construction method comprises the following steps: preparing a test sample solution; adopting a gas chromatographic method for determining a finger-print of bee wax by adopting a HP-5 chromatographic column, taking nitrogen as a carrier gas at the flow speed of 5mL/min, adopting a hydrogen flame detector as a detector and increasing the temperature of column box by maintaining for 15min at 280 DEG C, under the conditions of detector temperature at 310 DEG C, sampling port temperature at 330 DEG C, split ratio at 20:1, hydrogen flow speed at 40mL/min, air flow speed at 30mL/min and sample volume at 1uL; taking common characteristic peaks of at least 10 batches of finger-prints as the characteristic peaks of a standard finger-print, wherein the standard finger-print contains at least 11 characteristic peaks; and performing similarity evaluation on the finger-print by computer-assisted similarity evaluation software. The method and the standard finger-print provided by the invention can be used for comprehensively and accurately evaluating the quality of the bulk drug bee wax and are beneficial to the guaranteeing for the quality of the bee wax raw material and the clinical effect.
Description
technical field [0001] The invention relates to a method for detecting beeswax medicine, in particular to a method for constructing a beeswax GC standard fingerprint and the beeswax GC standard fingerprint obtained by the method. Background technique [0002] Chinese medicine fingerprint refers to the spectrum that can mark the common peaks of its characteristics obtained after proper processing of raw materials, decoction pieces, finished products, and semi-finished products of traditional Chinese medicine by using certain analysis methods. The fingerprint of traditional Chinese medicine can objectively reveal and reflect the integrity and characteristics of the internal quality of traditional Chinese medicine, and can be used to evaluate the authenticity, effectiveness, stability and consistency of traditional Chinese medicine. The clinical curative effect of traditional Chinese medicine is not determined by the effect of a single active ingredient or the simple addition o...
Claims
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Login to View More IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 罗春黄翔张利芳
Owner GUANGZHOU BAIYUNSHAN PHARM CO LTD