Method for simultaneously measuring content of six ingredients in canarium pimela leenh. leaves

A technology of black olive leaf and content, which is applied in the directions of measuring devices, material separation, and analysis of materials, can solve the problems of simultaneous determination of high performance liquid chromatography and other problems in unpublished records, and achieves high detection precision and accuracy, high separation efficiency, good repeatability

Active Publication Date: 2017-05-24
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of content determination, there is no high-performance liquid chromatography method for the simultaneous determination of the six components of chlorogenic acid, vitexin, isovitexin, hyperin, rutin and quercetin.

Method used

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  • Method for simultaneously measuring content of six ingredients in canarium pimela leenh. leaves
  • Method for simultaneously measuring content of six ingredients in canarium pimela leenh. leaves
  • Method for simultaneously measuring content of six ingredients in canarium pimela leenh. leaves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1 Sample solution preparation

[0036] Dry and crush the olive leaves, and pass through a No. 2 sieve. Accurately weigh 0.5g of black olive leaf powder in a stoppered Erlenmeyer flask, accurately add 50mL of 50% methanol aqueous solution (V / V), soak in the plug for 30min, then ultrasonicate for 30min, let stand to cool, and weigh. Add 50% aqueous methanol (V / V) to make up for weight loss, shake well, and filter. Take the filtrate and pass it through a 0.22 μm microporous membrane to obtain the sample solution.

[0037] 2. Preparation of reference solution

[0038] Accurately weigh the appropriate amount of chlorogenic acid, vitexin, isovitexin, hyperoside, rutin and quercetin reference substance, add 50% methanol aqueous solution (V / V) to ultrasonically dissolve, shake to a constant volume, that is A mixed reference solution was obtained.

[0039] 3. Chromatographic conditions

[0040] The chromatographic column is Agilent Zorbax SB-C 18 (250*4.6mm, 5μm); the flow...

Embodiment 2

[0077] Using the high-performance liquid chromatography method described in the present invention, the six components of chlorogenic acid, vitexin, isovitexin, hyperoside, rutin and quercetin in 10 batches of olive leaves were simultaneously Determination.

[0078] The experimental results are shown in Table 7:

[0079]

[0080] The test results showed that the relative content of chlorogenic acid components in black olive leaves was the highest. Different batches of black olive leaves contained six components of chlorogenic acid, vitexin, isovitexin, hyperin, rutin and quercetin. The content of each ingredient varies. The invention establishes a simple and effective content determination method, which is beneficial to better control the quality of black olive leaves and provides test basis for its further development.

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Abstract

The invention discloses a method for simultaneously measuring the content of six ingredients in canarium pimela leenh. leaves. The method comprises the following steps: 1, preparation of a sample solvent: drying and crushing the canarium pimela leenh. leaves, performing ultrasonic extraction on the crushed canarium pimela leenh. leaves, and performing volume fixation and filtering to obtain the sample solvent; 2, preparation of a reference solvent, wherein the reference solvent is prepared from known amounts of chlorogenic acid, vitexin, isovitexin, hyperoside, rutin and quercetin; 3, chromatographic analysis: performing high performance liquid chromatographic analysis on the sample solvent and the reference solvent, and calculating the content of the chlorogenic acid, the vitexin, the isovitexin, the hyperoside, the rutin and the quercetin in the canarium pimela leenh. leaves. According to the method for simultaneously measuring the content of the six ingredients in the canarium pimela leenh. leaves, main active ingredients of the canarium pimela leenh. leaves can be directly, rapidly and effectively measured, high detection precision, accuracy and reproducibility are ensured, and a strong scientific basis is provided for further development of the canarium pimela leenh. leaves.

Description

technical field [0001] The invention relates to the field of determination of black olive leaves, in particular to a method for simultaneously measuring the contents of six components in black olive leaves. Background technique [0002] Black olive (Canarium pimela Leenh.), also known as black olive, is an evergreen tree species used for woody oil and fruit. It belongs to the genus Olivaceae in plant classification. It is native to South China and widely distributed in Vietnam. And other Southeast Asian countries, its roots, leaves, and seeds can be used as medicine, and folks often use it to treat rheumatism, low back pain, numb hands and feet, stomach pain, and soup burns. [0003] Black olive leaves are easy to get and have high economic benefits. In the previous research of our research group, through methods such as rat isolated vascular ring perfusion, carotid artery cannulation and isolated heart perfusion, it was found that the extract of olive leaf had a rapid and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吕竹芬董艳芬梁燕玲方小爱
Owner GUANGDONG PHARMA UNIV
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