Nutritional compositions containing phosphatidylserine powder
A technology of phosphatidylserine and composition, applied in the field of PS preparations, which can solve the problems of manufacturers, increased PS degradation and oxidation, logistics problems, etc.
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Embodiment 1
[0074] Preparation of PS powder using single phase reaction (Sample 1) . 140 g of an aqueous emulsion containing ethanol-purified soybean lecithin was mixed with a solution containing 41 gr of L-serine, 35 ml of acetate buffer, and 0.55 gr of phospholipase-D enzyme, and stirred at 40°C. After stirring for 36 hours, the solution was filtered through a Buchner filter to remove all water-soluble components. Disperse the solid in 140 ml of purified water for 30 minutes, and filter again. The wet solid was dried in a vacuum dryer to obtain a dry PS powder with a PS concentration of 58.8% as measured by HPLC. The dry powder is ground with 2% silica to obtain a free-flowing powder, where more than 20% (w / w) of the powder has a particle size of 500 microns or more.
Embodiment 2
[0076] Sieving sample 1 (preparing sample 2) . Sample 1 was sieved through a 250 micrometer screen to obtain a powder in which 97% (w / w) of the powder has a particle size of 250 micrometers or less.
Embodiment 3
[0078] Purification of sample 1 using alcohol solvent (preparation of sample 3) . 10 gr of sample 1 (before drying) was dispersed in 40 ml of ethanol, and the solution was stirred for 30 minutes. The solution was filtered through a Buchner filter to separate solids and filtrate. Collect the solids from the filter and repeat the same process two more times. The wet solid is dried in a vacuum dryer to obtain a dry PS powder. The dry powder is ground with 2% silica to obtain a free-flowing powder, where more than 20% (w / w) of the powder has a particle size of 500 microns or more.
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