Fermented feed for dairy cows and preparation method thereof
A technology of fermented feed and fermented raw materials, applied in animal feed, animal feed, applications, etc., can solve the problems of restricting the widespread use of apple pomace, mycotoxin pollution, stinking and blackening, etc., to achieve the removal of bad smell, reduce the use of antibiotics, The effect of optimizing milk quality
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Embodiment 1
[0035] A fermented feed for dairy cows and a preparation method thereof, the specific steps are as follows:
[0036] 1) Preparation of seed solution
[0037] Slant culture: under sterile conditions, Saccharomyces cerevisiae, Aspergillus niger and Bacillus subtilis were respectively inoculated in the potato slant medium, and cultured at a constant temperature of 30°C for 48 hours.
[0038] The composition of the potato slant medium is as follows: 150 g of potatoes, 10 g of glucose, 20 g of agar, 1000 ml of water, natural pH, and sterilized at 121° C. for 30 minutes.
[0039] Seed liquid culture: under aseptic conditions, wash the activated slant strains with physiological saline, inoculate them into the seed medium, and culture them in constant temperature shake flasks at 30°C and 200r / min for 48 hours to obtain brewing Yeast seed liquid, Aspergillus niger seed liquid and Bacillus subtilis seed liquid; the composition of the seed liquid culture medium: 250g of potatoes, 20g of...
Embodiment 2
[0053] A fermented feed for dairy cows and a preparation method thereof, the specific steps are as follows:
[0054] 1) Preparation of seed solution
[0055] Slant culture: under sterile conditions, Saccharomyces cerevisiae, Aspergillus niger and Bacillus subtilis were respectively inoculated in the potato slant medium, and cultured at a constant temperature of 30°C for 48 hours. The composition of the potato slant medium is as follows: 150 g of potatoes, 10 g of glucose, 20 g of agar, 1000 ml of water, natural pH, and sterilized at 121° C. for 30 minutes.
[0056] Seed liquid culture: Under sterile conditions, wash the activated slant strains with physiological saline and inoculate them into the seed medium, and culture them in constant temperature shake flasks at 30°C and 200r / min for 48 hours to obtain Saccharomyces cerevisiae Seed liquid, Aspergillus niger seed liquid and Bacillus subtilis seed liquid; the composition of the seed liquid culture medium: 250g of potatoes, 2...
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