Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of genic polymorphic site and kit

A technology of gene polymorphism and site, applied in the field of medicine

Inactive Publication Date: 2017-05-31
JIANGSU CANCER HOSPITAL
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ICOS gene polymorphism site rs1559931 is a SNP site located on the 3'-UTR of the ICOS gene. At present, no studies have shown that the polymorphism site rs1559931 of the ICOS gene is related to the efficacy of chemotherapy drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of genic polymorphic site and kit
  • Application of genic polymorphic site and kit
  • Application of genic polymorphic site and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A kit and method for analyzing the genotype of the ICOS gene polymorphism site rs1559931 based on the single base extension-MALDI / TOF / MS method or predicting the efficacy of chemotherapy in patients with advanced colorectal cancer.

[0060] 1. The composition of the kit:

[0061] 1. Primers for detecting the genotype of ICOS gene polymorphism site rs1559931;

[0062] Primers for amplifying polymorphic loci:

[0063] SEQ ID No. 1: 5'-ACGTTGGATGGATTTGGCTAGAAAGATTC-3'

[0064] SEQ ID No.2: 5'-ACGTTGGATGCATGCAGACAGGAAGTAGAG-3'

[0065] Primers for single base extension of polymorphic loci:

[0066] SEQ ID No.3: 5'-CTTGGCTAGAAAGATTCTTAAATAT-3'

[0067] 2. PCR amplification enzyme and corresponding buffer;

[0068] 3. dNTPs;

[0069] 4. PCR product purification enzyme;

[0070] 5. Single base extension reaction enzyme and corresponding buffer;

[0071] 6. Enzyme purification of single base extension products.

[0072] 2. Genotype detection and curative effect predict...

Embodiment 2

[0151] Using the method provided in Example 1, the blood samples of 113 patients with advanced colorectal cancer were tested to obtain the genotype of the patient's ICOS gene polymorphism site rs1559931. These patients were given chemotherapy for 4 months, and the chemotherapy drug was oxaliplatin. According to the genotype of the polymorphic site rs1559931, the curative effect of chemotherapy in advanced colorectal cancer can be predicted: the number of people with genotype GG is 89, which predicts the chemotherapy effect of patients with advanced colorectal cancer is good; the number of people with genotype GA is 23, and the number of people with prediction The chemotherapy effect was poor, the results are shown in Table 1.

[0152] Table 1: The correlation between rs1559931 genotype and chemotherapy efficacy in patients with advanced colorectal cancer analyzed by unconditional Logistic regression model

[0153]

[0154] The results showed that the detection results of p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of an ICOS genic polymorphic site rs1559931, a detection primer thereof and a kit. Experiments prove that an advanced colorectal cancer chemotherapy effect can be analyzed according to a polymorphic site rs1559931 genotype, and when the genotype is GG, it is predicted that the chemotherapy effect on an advanced colorectal cancer patient is good, and when the genotype is GA, the chemotherapy effect is poor. The invention provides a primer group and the kit for detecting the ICOS genic polymorphic site rs1559931 and a detecting method, the polymorphic site rs1559931 can be accurately determined, the advanced colorectal cancer chemotherapy effect can be analyzed, and an analysis result conforms to an expectation.

Description

technical field [0001] The invention relates to the field of medicine, and relates to the use and kit of a gene polymorphism site, in particular to the use of the ICOS gene polymorphism site rs1559931 and its detection primers and kit. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignancies. Worldwide, the incidence of CRC ranks third and fourth among malignant tumors in males and females, and the mortality rate ranks fourth and fifth, respectively. In recent years, the incidence of CRC in my country has shown an obvious upward trend, especially in developed coastal areas and large and medium-sized cities. According to statistics, the annual average number of new CRC cases in my country reaches 130,000, accounting for about 12% to 15% of systemic tumors, and continues to rise with an average annual growth rate of 4%. Due to its rapid development, high degree of malignancy, and difficult treatment, CRC has been paid more and more attenti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/106C12Q2600/156
Inventor 王蕾汪维鹏
Owner JIANGSU CANCER HOSPITAL