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Myosin adenosine triphosphate staining method for frozen sections of skeletal muscle tissue

A technology of adenosine triphosphate and dyeing method, which can be used in disease diagnosis, instrument, biological material analysis, etc., can solve the problems of poor dyeing effect, time-consuming, waste of reagents, etc., and achieve the effect of convenience, saving reagents, and improving work efficiency.

Inactive Publication Date: 2019-02-01
FUJIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many problems in practical application: due to the repeated use of the dyeing solution, the dyeing effect is poor, and the reagents are required to be prepared immediately after use, and the pH of the solution should be precisely adjusted; however, the amount of reagents should not be too small for the dip dyeing method and the detection of pH value. , which is time-consuming and a waste of reagents

Method used

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  • Myosin adenosine triphosphate staining method for frozen sections of skeletal muscle tissue
  • Myosin adenosine triphosphate staining method for frozen sections of skeletal muscle tissue
  • Myosin adenosine triphosphate staining method for frozen sections of skeletal muscle tissue

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Embodiment 1

[0037] Myosin adenosin triphosphate (ATPase) staining kit for frozen sections of skeletal muscle tissue, the kit contains the following components:

[0038] (1) CaCl 2 Buffer solution (No. 1 solution): 0.1mol / L glycine solution, 0.1mol / L NaCl solution, 1 mol / LCaCl 2 , 0.1 mol / L NaOH, adjust the pH value to 9.5±0.01 with 0.1 mol / L NaOH;

[0039] (2) Acetate buffer solution (No. 2 solution): 0.2 mol / L sodium acetate, 0.2 mol / L acetic acid, adjust the pH value to 4.6±0.01 with 0.1mol / L HCl;

[0040] (3) Substrate incubation solution (No. 3 solution): Take the CaCl in No. 1 solution 2 10ml of buffer solution, 25mg of ATP-disodium salt, adjust the pH to 9.4±0.01 with 0.1mol / L NaOH;

[0041] (4) 2wt.% cobalt chloride solution (No. 4 solution);

[0042] (5) Ammonium sulfide solution (No. 5 solution): Ammonium sulfide working solution: 1wt% ammonium sulfide solution, freshly prepared before use.

[0043] No. 1-3 liquids should be stored frozen at -20°C, -35°C or -80°C; liquids 4...

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Abstract

The invention provides a skeletal muscle tissue frozen section myosin adenosine triphosphatase (ATPase) dyeing kit which comprises a CaCl2-containing buffer solution, an acetate buffer solution, a substrate incubation solution, 2wt% of cobalt chloride solution and 1wt% of ammonium sulfide solution. In the invention, a skeletal muscle tissue frozen section myosin adenosine triphosphatase dyeing kit is established, thus, the use is convenient, the working efficiency is improved, and the reporting period of a case is shortened; moreover, the reagent is saved, the cost is remarkably lowered, and a stable and satisfactory result is obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a myosin adenosin triphosphate (ATPase) staining kit for frozen sections of skeletal muscle tissue. Background technique [0002] Myosin ATPase is localized in skeletal muscle. Skeletal muscle fibers are divided into type I muscle and type II muscle. Type II muscle fibers are divided into IIA and IIB. Under normal circumstances, types I, IIA, and IIB are distributed in a mosaic pattern. Pathological changes should be considered in the following cases of ATPase staining: grouping of muscle fiber types, dominance of a certain type of fiber, and disordered distribution of a certain type of fiber; atrophy or hypertrophy of a certain type of fiber, changes in the size and shape of muscle fibers such as rounded or cord-like; Undifferentiated type IIC myofibers appear. Normal skeletal muscle of infants before 4-5 years old has type IIC muscle fibers, which are undifferentiated ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6887G01N2333/46G01N2333/4712G01N2800/10
Inventor 高美钦刘文文陈裕庆晋雯张文敏
Owner FUJIAN MEDICAL UNIV
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