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A new nanochip for mass spectrometry detection and its preparation and application

A mass spectrometry and nanotechnology technology, applied in the field of mass spectrometry detection, can solve the problems of low detection sensitivity of biomolecules, low signal-to-noise ratio of detection signals, uneven matrix distribution, etc., to avoid strong background noise, good reproducibility, and low cost. Effect

Active Publication Date: 2019-07-26
亿纳谱(浙江)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, MALDI has the disadvantages of low detection sensitivity for low-abundance biomolecules, low signal-to-noise ratio of detection signals for low-molecular-weight biomolecules, and uneven matrix distribution.
At the same time, the introduction of the matrix also leads to the existence of matrix peaks in the low molecular weight region, which greatly limits the application of this method in the analysis of small molecules

Method used

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  • A new nanochip for mass spectrometry detection and its preparation and application
  • A new nanochip for mass spectrometry detection and its preparation and application
  • A new nanochip for mass spectrometry detection and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: This chip is used for the detection of glucose standard substance

[0045] (1) Instruments and reagents: AB 5800MALDI TOF laser desorption ionization mass spectrometer (reflection mode, positive ion detection), Millipore Milli-Q system pure water instrument, GeSiM spotting instrument.

[0046] (2) Dilute the glucose sample to be detected by 0-10000 times.

[0047] (3) Apply samples on the novel nanochip and dry at room temperature.

[0048] (4) Glucose molecules were analyzed by mass spectrometry.

[0049] The results are shown in figure 2 .

Embodiment 2

[0050] Embodiment 2: This chip is used for the detection of lysine standard substance

[0051] (1) Instruments and reagents: AB 5800MALDI TOF laser desorption ionization mass spectrometer (reflection mode, positive ion detection), Millipore Milli-Q system pure water instrument, GeSiM spotting instrument.

[0052] (2) Dilute the lysine sample to be detected by 0-10000 times.

[0053] (3) Apply samples on the novel nanochip and dry at room temperature.

[0054] (4) Perform mass spectrometry analysis.

Embodiment 3

[0055] Example 3: The chip is used for the qualitative detection of small molecules in serum

[0056] (1) Instruments and reagents: AB 5800MALDI TOF laser desorption ionization mass spectrometer (nitrogen laser, wavelength 355nm, reflection mode, positive ion detection), Millipore Milli-Q system pure water instrument, GeSiM spotting instrument, Data Explore software, OriginPro 2015 software.

[0057] (2) Dilute the serum by 0-10000 times.

[0058] (3) Spotting the diluted serum on the novel nanochip for detection.

[0059] (5) Analyze the obtained mass spectrometry results.

[0060] The results are shown in image 3 .

[0061] The nanochip in the present invention can realize quantitative and qualitative detection of biomolecules in mass spectrometry; and in mass spectrometry, there will be no strong background noise and heterogeneity brought by traditional matrix detection, so it can be detected more effectively Small molecules in the sample for better analysis. The chip ...

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Abstract

The invention discloses a nano chip for mass spectrum detection. SiO2 particles are arranged at a bottom layer of the nano chip, wherein the particle size of the SiO2 particles is uniform and is not larger than 1 micron, the SiO2 particles have smooth surfaces; the nano chip has a rough metallic surface layer comprising gold, silver, platinum, palladium, aluminum, ferrum, titanium, copper, zinc, nickel, chromium, molybdenum, tin and barium. The invention also discloses a preparation method of the nano chip and application of the nano chip in mass spectrometry of a biological sample. The nano chip has better salt tolerance, and is applicable for quick, parallel and high-efficiency detection on practical biological samples. The nano chip disclosed by the invention has the advantages of simple preparation method, short preparation time, low economic cost and capability of being produced in batch, and therefore has a better application prospect and is worth of popularization and application.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection, in particular to a novel nano-chip for mass spectrometry detection and its preparation and application. Background technique [0002] Mass spectrometry (MS) is an analytical method that measures the mass-to-charge ratio (mass-to-charge ratio) of ions. It converts biomolecules into gas-phase ions through an ionization source, and then uses the electric field and magnetic field of the mass spectrometer to separate ions with a specific mass-to-charge ratio (m / z), and collects the separated ions through an ion detector to determine the ions The z-values ​​are then analyzed and identified by data retrieval to identify unknown molecules. Mass spectrometry is used for biomolecular identification with high precision, short analysis time, and the ability to process many samples simultaneously. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a soft ionization techniq...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 钱昆孙旭明
Owner 亿纳谱(浙江)生物科技有限公司