How to obtain the brightness and concentration of each species

Abstract

An embodiment of the invention discloses a method for obtaining brightness and concentration of various species. The method comprises steps as follows: laser pulses are continuously transmitted m times towards a solution to excite molecules of the species to emit photons, the photons are received through a detector, and photon stream data are obtained; the number of photons emitted through excitation of the laser pulses is calculated on the basis of the obtained photo stream data, so that the frequency at which a certain number of the photons are excited is obtained; the occurrence probability of at least two photon excitation conditions is determined according to the frequency; an equation set is established according to the quantitative relation between the occurrence probability of the at least two photon excitation conditions and brightness and concentration of the species, and the brightness and the concentration of the species in the solution are calculated. The problem that the brightness and the concentration of multiple species with slight fluorescence lifetime difference cannot be obtained is solved, and the benefit of obtaining the brightness and the concentration of the species is achieved.

Description

technical field [0001] The embodiments of the present invention relate to the field of fluorescence analysis, and in particular to methods for acquiring brightness and concentration of various species. Background technique [0002] With the rapid development of the field of optical detection, fluorescence correlation spectroscopy has been reused in the detection of material properties and intermolecular interactions. [0003] Fluorescence Correlation Spectroscopy (FCS) is a commonly used method for studying molecular diffusion and chemical reaction kinetics. -15 L) The fluctuation of fluorescence intensity of fluorophores due to Brownian motion or chemical reaction is an important single-molecule detection technique. [0004] However, when there are multiple species in the system, the traditional FCS analysis method obtains the summation of the behaviors of all species, and cannot distinguish species, and thus cannot obtain information on material properties. Although para...

AI Technical Summary

Problems solved by technology

[0004] However, when there are multiple species in the system, the traditional FCS analysis method obtains the sum of the behaviors of all species, and cannot distinguish species, so it is impossible to obtain information on material properties

Although parameters such as fluorescence lifetime can be used to distinguish fluorescent species and obtain the brightness and concentration of each species, it is only applicable to the situation where the fluorescence lifetimes of species are greatly different, and cannot be obtained when the fluorescence lifetimes of multiple species are different.

Images