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Application of aspartic acid serving as neomycin sulfate synergist

A technology of neomycin sulfate and aspartic acid, which is applied in the field of medicine, can solve problems such as malnutrition, metabolic disorders, and abnormal protein formation, and achieve the effects of reducing the amount of use, enhancing the bactericidal effect, and increasing the survival rate of fish

Inactive Publication Date: 2017-06-20
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In aquaculture, aspartic acid is a non-essential amino acid. Although the fish body can synthesize aspartic acid by itself, the lack of aspartic acid will cause most of the proteins in the body to fail to form normally, and cause the body's nutritional deficiency. Symptoms such as adverse and metabolic disorders

Method used

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  • Application of aspartic acid serving as neomycin sulfate synergist
  • Application of aspartic acid serving as neomycin sulfate synergist
  • Application of aspartic acid serving as neomycin sulfate synergist

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Aspartic acid increases the ability of neomycin sulfate to kill bacteria in vitro

[0024] 1. In vitro experimental methods of bacterial culture and survival rate

[0025] (1) Pick a single colony of Aeromonas hydrophila and inoculate it in 100mL LB medium, and culture it with shaking at 28°C overnight;

[0026] (2) Take an appropriate amount of bacteria liquid at 12000rpm for 5min to collect the bacteria by centrifugation, wash the bacteria twice with normal saline with a mass concentration of 0.85%, and suspend them in M9 medium containing 10mM sodium acetate, adjust the OD600 value to 0.6;

[0027] (3) Add the corresponding metabolites and appropriate amount of antibiotics to the test tube, add M9 medium to the 5mL system, and incubate at 28°C for 6 hours;

[0028] (4) At a specific time point, take a 100uL sample, use the serial dilution method, take 10uL for spot plate counting, and calculate the bacterial cfu / mL (colony forming unit / ml). The samples without antibiotics and...

Embodiment 2

[0036] Aspartic acid increases the ability of neomycin sulfate to eliminate bacteria in the body

[0037] 1. Fish infection and bacteria removal experiment: Aeromonas hydrophila Ah-479 is a highly virulent strain, Qihe crucian carp is injected intraperitoneally with 100μL 1.0×10 7 With a bacterial suspension of cfu / mL, 24 hours later, Qihe crucian carp was randomly divided into 4 groups, each with 6 tails, namely: saline control group, metabolite group, antibiotic group, and antibiotic and metabolite group with corresponding concentrations. The dosage of aspartic acid is 50 mg / kg, and the dosage of neomycin sulfate is 40 mg / kg. After 24 hours of treatment, the liver, kidney, spleen and other tissues of the fish were taken and weighed, and 1 mL of normal saline was added to fully grind the homogenate, and the bacteria content in the tissues (cfu / g) was detected by plate counting.

[0038] 2. Aspartic acid combined with neomycin sulfate can significantly eliminate Aeromonas hydrophil...

Embodiment 3

[0041] Aspartic acid combined with neomycin sulfate improves the survival rate of bacteria in fish infection

[0042] Aspartic acid combined with neomycin sulfate to improve fish body treatment effect

[0043] The median lethal dose of Ah-479 (LD 50 ) Is 1.55×10 6 cfu / g, when the injection dose is 100μL 5.0×10 7 At the dose of cfu / mL, one hour after the challenge, injection or oral administration is used for treatment. Each administration method is divided into 4 groups: the control group is normal saline, the aspartic acid group is 50mg / kg, the neomycin sulfate group is 40mg / kg and 50mg / kg aspartic acid+40mg The neomycin / kg neomycin sulfate group was used at the same time, all injections or oral medications were 100 μL / tail, and each group had 20 Qihe crucian carp. After bacterial infection, observe the morbidity and death of fish every 12h for 7 days. Drug protection rate=[1-(mortality rate of administration group / mortality rate of control group)]×100%.

[0044] The results of t...

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Abstract

The invention discloses application of aspartic acid serving as a neomycin sulfate synergist. According to application disclosed by the invention, the aspartic acid is found to serve as the synergist of antimicrobial medicine for the first time and especially serve as a synergist of neomycin sulfate to kill aeromonas hydrophila. In-vitro and in-vivo experiments show that combined utilization of the aspartic acid and the neomycin sulfate can greatly improve a killing or clearing effect on the aeromonas hydrophila, a treating effect on aeromonas hydrophila infection of fish can be improved by injecting or orally taking the two compositions, and the fish survival rate is remarkably improved. Therefore, the aspartic acid disclosed by the invention can serve as the synergist of the neomycin sulfate, can be combined with the neomycin sulfate to be applied to preparation of compound antibacterial medicine, not only improves a pesticide effect of the antibacterial medicine, but also remarkably reduces the use amount of medicine and further reduces side effects of the medicine and medicine residue.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of aspartic acid as an antibacterial drug neomycin sulfate synergist. Background technique [0002] Aeromonas hydrophila is widespread in natural environments such as rivers, lakes, ponds, food, animals and humans. Aeromonas hydrophila is a conditional pathogen that can cause diseases in a variety of aquatic and terrestrial animals. It is a typical human-animal-fish comorbid pathogen. When the environmental conditions are bad, the body's resistance will decrease It can cause disease in cold-blooded animals and mammals such as fish, amphibians, reptiles, and cause bacterial hemorrhagic disease in aquatic animals, body surface ulcers, and mammalian diarrhea. It is a deadly source of infection for fish, reptiles and humans. . In order to prevent and treat diseases caused by pathogenic bacteria, antibiotics are often used. The long-term abuse of antibiotics h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7036A61K31/198A61P31/04
CPCA61K31/198A61K31/7036A61K2300/00
Inventor 孔祥会赵贤亮李莉陈鹤郭胜喜杨潇巴新霞
Owner HENAN NORMAL UNIV
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