Super-resolution biomolecular mass spectrometry imaging device and working method thereof

Abstract

The invention discloses a super-resolution biomolecular mass spectrometry imaging device and a working method thereof. The device is characterized by comprising: a laser, an optical modulator assembly, an optical element, a slide, a camera, a reflector and a mass spectrometer. The laser emitted by a laser is modulated by the optical modulator assembly, and is then focused on a target sample placed on the slide through the optical element, desorption ionization occurs to the target sample, then the sample enters the mass spectrometer to undergo detection, the optical modulator assembly consists of a coding aperture, by which the target sample at a corresponding pixel detection position is irradiated by laser, and the reflector and the camera are used for acquiring the targe sample information. The super-resolution biomolecular mass spectrometry imaging device provided by the invention adopts the mass spectrometry imaging technology based on the coding aperture and mass spectrometer, changes the inherent framework of existing lattice optical mechaical scanning, acquires super-resolution biomolecular microscopic mass spectrometry imaging, and has the advantages of high spatial resolution, high sensitivity, fast imaging speed and high signal-to-noise ratio.

Description

technical field [0001] The invention relates to the field of life science analysis, in particular to a super-resolution biomolecular mass spectrometry imaging device and a working method thereof. Background technique [0002] At present, the imaging method of the mass spectrometry imaging system routinely adopts the dot-matrix optical-mechanical scanning method. This type of imaging system has the following disadvantages: [0003] 1. Low spatial resolution: The focused spot of the laser is obtained by the optical system based on the slit. The laser spot has the problems of diffraction and spot expansion, and is affected by the optical-mechanical structure, so the structure is inevitably unstable. Therefore, when the laser resolves the target sample, it will inevitably diffract to adjacent samples, causing some target samples to be repeatedly analyzed and ionized, which will affect the spatial resolution of the mass spectrometry imaging system. [0004] 2. Slow imaging spe...

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Problems solved by technology

[0003] 1. Low spatial resolution: the focused spot of the laser is obtained by the slit-based optical system

The laser spot has the problems of diffraction and spot expansion. At the same time, it is affected by the structure of the optical machine, and the structure is inevitably unstable.

Therefore, when the laser resolves the target sample, it will inevitably diffract to adjacent samples, causing some target samples to be repeatedly analyzed and ionized, which will affect the spatial resolution of the mass spectrometry imaging system.

[0004] 2. Slow imaging speed: To generate a mass spectrometer image, laser analysis of millions of sample points is often required. The imaging speed is affected by the ion conversion efficiency, and the ionized samples have not been transferred and detected in time

As a result, the scanning speed of conventional mass spectrometry imaging is about 1 pixel / second, and each imaging process usually takes several hours, so it cannot meet the real-time monitoring of important chemical substances and metabolites in biological samples

[0005] 3. Low sensitivity: Due to the limited energy of a single laser, the desorption and ionization of the sample under a single laser irradiation is less, and it is inevitable to increase the number of exposures and intensity to increase the energy of laser irradiation

However, the increase of laser intensity and too many exposure times will cause the denaturation of the sample and affect the detection of the sample.

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