The invention belongs to the
biochemical detection field and particularly relates to a biochemical quantitative detection device and method based on photo-
thermal interference of
scattered light. According to the device and method, the problems that a micro-volume
rapid detection method of
colored compounds only can be utilized for carrying out qualitative and semiquantitative detection and can cause more false positive results, and developed pictures of to-be-detected matters acquired by equipment such as cameras and mobile phones are easily interfered by external light sources are solved. The device comprises a microchannel, a
laser device, a photoelectric
detector, a total reflection mirror and a computer, wherein the microchannel is formed in a microchannel fixing platform, the total reflection mirror which forms an included angle of 45 degrees with the horizontal plane is arranged above the microchannel, the
laser device and the photoelectric
detector are arranged on one side of the mirror surface of the total reflection mirror, the
laser device is horizontally arranged, and the photoelectric
detector is connected with the computer. By combining a
scattered light interferencetechnique with a photo-
thermal effect, the rapid quantitative detection of
colored matters is realized, and the volume of a sample required by the related detection method is small, so that the influences caused by external
environmental temperature are avoided.