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Biochemical quantitative detection device and method based on photo-thermal interference of scattered light

A photothermal interference and detection method technology, which is applied in the direction of measuring devices, color/spectral characteristic measurement, and analysis through chemical reactions of materials, can solve the problems of external light source interference in pictures, multiple false positive results, etc., and achieve greater The effect of laser intensity, small sample volume, and improved detection accuracy

Active Publication Date: 2018-06-05
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In order to solve the problem that the micro-volume rapid detection method of colored compounds in the prior art can only perform qualitative and semi-quantitative detection and there are many false positive results, and the picture of the substance to be tested after color development is obtained by using a camera, a mobile phone and other equipment is easily affected. The problem of external light source interference provides a detection device and method based on scattered light photothermal interference biochemical quantification

Method used

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  • Biochemical quantitative detection device and method based on photo-thermal interference of scattered light
  • Biochemical quantitative detection device and method based on photo-thermal interference of scattered light
  • Biochemical quantitative detection device and method based on photo-thermal interference of scattered light

Examples

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Effect test

Embodiment 1

[0034] Quantitative detection of nitrite ion:

[0035] 1. Preparation of standard solution: Dissolve sodium nitrite in deionized water to prepare NO concentrations of 0.5, 1, 2, 3, 4, 5 mg / L 2 - Standard solution; preparation of color developer: Take 0.0861 g of sulfanilamide, 0.634 g of citric acid and 0.0259 g of naphthaleneethylenediamine hydrochloride and dissolve them in ultrapure water at 45 ℃ ~ 50 ℃ in sequence, and stir until completely dissolved.

[0036] The standard solution and the chromogenic reagent were fully mixed and reacted in the centrifuge tube at a ratio of 5:1. After standing for 10 min for color development, 20 μL of the solution was passed into the sample area of ​​the microchannel.

[0037] 2. Selection and installation of detection device models: such as figure 1 As shown, choose the laser 1 that is complementary to the color of the chromogenic agent. In this example, a 532 nm all-solid-state laser is selected. A beam of light is emitted by the l...

Embodiment 2

[0042] Quantitative detection of divalent copper ions:

[0043] 1. Standard solution: Dissolve copper nitrate in deionized water to prepare Cu with concentrations of 0,0.5,1,2,3,4,5,10 mg / L 2+ Standard solution; preparation of chromogen: Weigh 100 mg 2,9-dimethyl-1,10-phenanthroline (C 14 h 12 N 2 1 / 2H 2 o) dissolved in 100 ml methanol (5.7). This solution is stable for one month. The prepared chromogen was stored in a 4ºC refrigerator away from light. Hydroxylamine hydrochloride divalent copper ion is reduced to cuprous ion, and in neutral or slightly acidic solution, cuprous ion reacts with 2,9-dimethyl-1,10-phenanthroline to form a yellow complex.

[0044] 2. Detection device design: the used laser wavelength is a blue light laser of 457nm, and others are consistent with the detection device in Embodiment 1, such as figure 1 shown.

[0045] 3. Obtain the backscattering interference pattern: use the CCD fixed on the optical platform, set the CCD to the continuous ...

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Abstract

The invention belongs to the biochemical detection field and particularly relates to a biochemical quantitative detection device and method based on photo-thermal interference of scattered light. According to the device and method, the problems that a micro-volume rapid detection method of colored compounds only can be utilized for carrying out qualitative and semiquantitative detection and can cause more false positive results, and developed pictures of to-be-detected matters acquired by equipment such as cameras and mobile phones are easily interfered by external light sources are solved. The device comprises a microchannel, a laser device, a photoelectric detector, a total reflection mirror and a computer, wherein the microchannel is formed in a microchannel fixing platform, the total reflection mirror which forms an included angle of 45 degrees with the horizontal plane is arranged above the microchannel, the laser device and the photoelectric detector are arranged on one side of the mirror surface of the total reflection mirror, the laser device is horizontally arranged, and the photoelectric detector is connected with the computer. By combining a scattered light interferencetechnique with a photo-thermal effect, the rapid quantitative detection of colored matters is realized, and the volume of a sample required by the related detection method is small, so that the influences caused by external environmental temperature are avoided.

Description

technical field [0001] The invention belongs to the field of biochemical detection, in particular to a detection device and method based on scattered light photothermal interference biochemical quantification. Background technique [0002] The color reaction is a chemical reaction that converts the measured component in the sample into a colored compound. Generally, by selecting an appropriate reagent, the ion to be measured is converted into a colored compound. According to the degree of color change and the relationship between the sample to be tested Perform semi-quantitative or quantitative analysis. The commonly used detection methods in color reaction mainly include colorimetry, spectrophotometry and so on. Although the colorimetric method is simple to operate and can be used for semi-quantitative analysis by naked eye observation, errors usually occur due to the differences in the biological vision and senses of different testers. Therefore, it is necessary to furth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/39G01N21/78
CPCG01N21/01G01N21/39G01N21/78
Inventor 张校亮陈小红李晓春于化忠
Owner TAIYUAN UNIV OF TECH
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