Calibration Method of Image Sensor Array in Wide Field of View and High Resolution Microscopic Imaging

Abstract

The invention discloses a method for calibrating an image sensor array in a wide-field high-resolution microscopic imaging, which includes: calibrating and correcting the vignetting effect on the image sensor array, so that all sub-fields of view in the image sensor array can Each pixel obtained in the scene with the same radiance has the same gray value; the spatial light modulator is used to encode the space-time joint structure to establish the correspondence between multiple feature points in the image space and the physical space ; Optically decode the space-time joint structure to obtain the pixel coordinates of multiple feature points on the image plane and the physical coordinates of multiple feature points on the spatial light modulator plane; according to the pixel coordinates of multiple feature points on the image plane and multiple The physical coordinates of the feature points on the plane of the spatial light modulator obtain a homography relationship, and a global coordinate mapping is obtained according to the homography relationship. The invention has the following advantages: meeting the requirement of obtaining wide field of view, high resolution and real-time microscopic images.

Description

technical field [0001] The invention belongs to the fields of structured light encoding, computational imaging and microscopic imaging, and in particular relates to a method for calibrating an image sensor array in wide-field high-resolution microscopic imaging. Background technique [0002] With the development of microbiology, people's requirements for microscopic imaging technology are increasing day by day. Obtaining wide-field, high-resolution, and real-time microscopic images is a prerequisite for conducting in-depth microbiological research. For example, for cancer research, observing the proliferation process of cancer cells requires high-resolution images, while observing the metastasis of cancer cells relies on a wide field of view. Furthermore, observing the rapid transmission of signals on neurons requires high-speed and real-time acquisition of microscopic images. [0003] Through a series of objective lens groups, the image seen by the microscope eyepiece can...

AI Technical Summary

Problems solved by technology

First of all, the limitation of the shallow depth of field of the microscopic objective lens makes it impossible to obtain three-dimensional information by changing the posture of the calibration object; secondly, it includes a multi-level objective lens imaging system like a curved surface, which makes it difficult to implement step-by-step calibration

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