Preparation method and application of fermentation products of edible and medical fungus

Abstract

The invention discloses a preparation method and application of fermentation products of an edible and medical fungus. On the basis of the characteristics of anabolism route of fermentation products (kaempferol and sinapic acid) of a medical fungus (pycnoporus cinnabarius), seeds of staphylea bumalda are added in the solid fermentation phase, annexin is added in a special fermentation phase, the content of kaempferol and sinapic acid in the liquid fermentation product of pycnoporus cinnabarius is effectively increased; and a novel method is provided for obtaining a product rich in kaempferol and sinapic acid. The edible and medical fungus fermentation product is rich in kaempferol and sinapic acid, can be used to produce health care products and animal feeds, and can also be applied to health care products and animal feeds.

Description

technical field

[0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to a preparation method and application of an edible and medicinal fungus fermentation product. Background technique

[0002] Edible and medicinal fungi are a class of large fungi with edible or medicinal value. In recent years, they have been increasingly favored by people because they are rich in various nutrients and biologically active substances.

[0003] Trametes cinnabarina (Jacq.) Fr. is a Basidiomycota Basidiomycetes Polyporacea organism, the fruiting body is sessile, the cap is corky, semicircular or fan-shaped and the base is narrow, and it has heat-clearing, dehumidifying and anti-inflammatory properties. , detoxification, and more used as traditional Chinese medicine. At present, among the bio-fermentation products of Rhodotrametes strains, the polysaccharides with tumor-inhibiting effects have attracted more attention, and studi...

Examples

preparation example Construction

[0046] Annexin preparation:

[0047] Put the sulphurite into liquid nitrogen, take it out after 10min-15min for crushing, put the crushed tissue in Tris-HCl buffer containing 5mmol / L EDTA (Tris-HCl buffer pH7.4, concentration 10mmol / L ), homogenate for 3 minutes, then centrifuge the homogenate at 4°C and 8000rmp for 20 minutes, discard the precipitate, and freeze-dry the supernatant into a freeze-dried powder; dissolve the freeze-dried powder in 0.05mol / LTris-HCl buffer , placed in a dialysis bag with a molecular weight exclusion value of 6kDa, and dialyzed against 0.05mol / LTris-HCl buffer solution at 4°C overnight; after the dialyzed sample was centrifuged to remove the precipitate, it was loaded on a well-balanced DEAECellulose DE-52 anion exchange column , use 0.05mol / L Tris-HCl buffer to pass through the column, the flow rate is 0.4mL / min, 6mL per tube is automatically collected, and the corresponding sample components are detected and collected at ultraviolet 280nm. After...

Embodiment 1

[0049] 1. Material preparation

[0050] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0051] Inoculate the Rhodotrametes strains preserved on the slant onto the PDA plate culture medium and activate and cultivate them at 22° C. for 10 days to obtain the activated Rhodotramets strains.

[0052] Liquid seed medium: glucose 12g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0053] The solid medium is composed of the following mass percentage components: K 2 HPO 4 0.1%, MgSO 4 0.1% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0054] The composition of the liquid fermentation medium is: based on 1L liquid fermentation medium, glucose 25g, peptone 6g, yeast powder 5g, KH 2 PO 4 1.0g, MgSO 4 0.5g and the rest of water, with natural pH, sterilized at 121°C for 20m...

Embodiment 2

[0076] 1. Material preparation

[0077] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0078] Inoculate the rhizome strains preserved on the slant on the PDA plate culture medium and activate and culture them at 30° C. for 3 days to obtain the activated rhizome strains.

[0079] Liquid seed medium: glucose 5g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0080] The solid medium is composed of the following mass percentage components: K 2 HPO 4 0.2%, MgSO 4 0.05% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0081] The liquid fermentation medium is the same as in Example 1.

[0082] 2. Preparation of medicinal bacteria fermentation products:

[0083] (1) Preparation of liquid seed solution of Rhodotrametes: Dilute 1cm 2 Inoculate the bacterium blocks of rhiz...

technical field

[0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to a preparation method and application of an edible and medicinal fungus fermentation product. Background technique

[0002] Edible and medicinal fungi are a class of large fungi with edible or medicinal value. In recent years, they have been increasingly favored by people because they are rich in various nutrients and biologically active substances.

[0003] Trametes cinnabarina (Jacq.) Fr. is a Basidiomycota Basidiomycetes Polyporacea organism, the fruiting body is sessile, the cap is corky, semicircular or fan-shaped and the base is narrow, and it has heat-clearing, dehumidifying and anti-inflammatory properties. , detoxification, and more used as traditional Chinese medicine. At present, among the bio-fermentation products of Rhodotrametes strains, the polysaccharides with tumor-inhibiting effects have attracted more attention, and studi...

Examples

preparation example Construction

[0046] Annexin preparation:

[0047] Put the sulphurite into liquid nitrogen, take it out after 10min-15min for crushing, put the crushed tissue in Tris-HCl buffer containing 5mmol / L EDTA (Tris-HCl buffer pH7.4, concentration 10mmol / L ), homogenate for 3 minutes, then centrifuge the homogenate at 4°C and 8000rmp for 20 minutes, discard the precipitate, and freeze-dry the supernatant into a freeze-dried powder; dissolve the freeze-dried powder in 0.05mol / LTris-HCl buffer , placed in a dialysis bag with a molecular weight exclusion value of 6kDa, and dialyzed against 0.05mol / LTris-HCl buffer solution at 4°C overnight; after the dialyzed sample was centrifuged to remove the precipitate, it was loaded on a well-balanced DEAECellulose DE-52 anion exchange column , use 0.05mol / L Tris-HCl buffer to pass through the column, the flow rate is 0.4mL / min, 6mL per tube is automatically collected, and the corresponding sample components are detected and collected at ultraviolet 280nm. After...

Embodiment 1

[0049] 1. Material preparation

[0050] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0051] Inoculate the Rhodotrametes strains preserved on the slant onto the PDA plate culture medium and activate and cultivate them at 22° C. for 10 days to obtain the activated Rhodotramets strains.

[0052] Liquid seed medium: glucose 12g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0053] The solid medium is composed of the following mass percentage components: K 2 HPO 4 0.1%, MgSO 4 0.1% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0054] The composition of the liquid fermentation medium is: based on 1L liquid fermentation medium, glucose 25g, peptone 6g, yeast powder 5g, KH 2 PO 4 1.0g, MgSO 4 0.5g and the rest of water, with natural pH, sterilized at 121°C for 20m...

Embodiment 2

[0076] 1. Material preparation

[0077] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0078] Inoculate the rhizome strains preserved on the slant on the PDA plate culture medium and activate and culture them at 30° C. for 3 days to obtain the activated rhizome strains.

[0079] Liquid seed medium: glucose 5g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0080] The solid medium is composed of the following mass percentage components: K 2 HPO 4 0.2%, MgSO 4 0.05% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0081] The liquid fermentation medium is the same as in Example 1.

[0082] 2. Preparation of medicinal bacteria fermentation products:

[0083] (1) Preparation of liquid seed solution of Rhodotrametes: Dilute 1cm 2 Inoculate the bacterium blocks of rhiz...

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