Method for identifying and evaluating level of wilt resistance of sesame

A technology for sesame wilt and fusarium wilt, which is applied in the timely field of crop variety disease resistance evaluation, can solve problems affecting the monitoring of sesame disease resistance response process, incomplete manifestation of fusarium disease symptoms, and unfavorable observation of sesame disease, etc., and achieves identification results Stable and reliable, good scientific research application value, good repeatable effect
CN106967783AActive Publication Date: 2017-07-21HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
Publication Date
2017-07-21

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Abstract

The invention specifically relates to a method for identifying and evaluating the level of wilt resistance of sesame, belonging to the field of timely evaluation of the disease resistance of crop varieties. The method comprises the following steps: preparation of sesame plants; preparation of a conidium suspension used for dip dyeing; dipping of roots and inoculation of bacteria; identification and evaluation of the wilt resistance of a sesame variety; etc. The method provided by the invention can realize inoculation of sesame plants in different growth stages (from a stage of two pairs of true leaves to a squaring stage) on the basis of a mature root-dipping infection method. Meanwhile, since the concentration of a wilt strain for inoculation is easy to adjust, so detailed analysis can be carried out on both the pathogen infection process and the response process of sesame seedlings, the disease resistance of different sesame germplasms and the pathogenicity of different strains can be accurately identified and evaluated, a technical base is provided for effective control of sesame wilt, and reference is provided for control of other crop diseases. The method provided by the invention has good repeatability, stable and reliable identification results and good application value in scientific research.
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Description

technical field

[0001] The invention belongs to the field of crop variety disease resistance evaluation and in particular relates to a fusarium wilt resistance identification and evaluation method for sesame. Background technique

[0002] Sesame is an important characteristic oil crop in my country. Sesame Fusarium Wilt is mainly caused by Fusarium oxysporum Sesame Specialization ( Fusarium oxysporum f. sp. sesami , FOS) is also a worldwide sesame disease. Together with stem point blight, it is called the two major fungal diseases in sesame production. The annual incidence of sesame wilt is about 15%, and it can reach more than 30% in severe cases, which has a great impact on yield and quality.

[0003] In recent years, in order to detect the pathogenicity of the sesame-specific type of Fusarium oxysporum and evaluate the resistance level of sesame germplasm to Fusarium wilt, the Sesame Research Center of Henan Academy of Agricultural Sciences used the method of seed inoc...

Claims

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