Method for Molecular Typing of Pepper and Tomato Bacterial Scab fungus mlst

Abstract

The invention discloses a method for molecular typing of capsicum and tomato bacterial scab, and for the first time discloses an operation technique for molecular typing of capsicum and tomato bacterial scab by using a multi-locus sequence typing method (MLST). The invention extracts the genomic DNA of the pathogenic bacteria, screens, amplifies, detects and sequences through seven housekeeping genes. DNAMAN software was used to perform multiple sequence alignment and analysis on the sequencing results to obtain the sequence type (ST) of the strain. Sequence type analysis was performed online using the eBURST V3 program, which can effectively distinguish the four pathogenic bacteria. The 7 housekeeping gene combinations and amplification primers used in this typing technique were adopted for the first time, and the PCR reaction system and conditions were self-optimized. This technical process can be used for taxonomic identification and genetic diversity research of pepper and tomato bacterial scab.

Description

technical field [0001] The present invention relates to plant pathogenic bacteria molecular typing technique, be specifically related to pepper and tomato bacterial scab (Bacterial Spot caused by Xanthomonads ) method for typing. Background technique [0002] Capsicum and tomato scab is a bacterial disease that commonly occurs on Solanaceae plants. The pathogen mainly damages the leaves, stems, and fruits of peppers and tomatoes, especially on leaves. In the early stage of the disease, small water-soaked, yellow-green spots are formed on the leaves, and after enlargement, they become round or irregular, dark brown, with raised edges and sunken centers, rough and scab-like. In severe cases, the leaves turn yellow, dry, crack, and fall off early. The stems and fruiting stems are diseased, and water-soaked spots are formed at the beginning, and gradually develop into short brown streaks. The lesions are suberized and raised, and the longitudinal fissures are ulcer-like scabs...

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So far, no research has been reported on the multilocus sequence typing of pepper and tomato bacterial scab

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