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Pepper and tomato bacterial streptomyces scabies MLST molecular typing method

A technology of scab bacteria and molecular typing, applied in the direction of biochemical equipment and methods, instruments, electronic digital data processing, etc., can solve problems that have not been reported

Active Publication Date: 2017-07-21
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no research has been reported on the multilocus sequence typing of pepper and tomato bacterial scab

Method used

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  • Pepper and tomato bacterial streptomyces scabies MLST molecular typing method
  • Pepper and tomato bacterial streptomyces scabies MLST molecular typing method
  • Pepper and tomato bacterial streptomyces scabies MLST molecular typing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Extraction of bacterial genomic DNA

[0037] Bacterial genomic DNA was extracted according to the instructions of the DNA extraction kit (spin column type).

[0038] 2. The housekeeping gene is determined

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Abstract

The invention discloses a pepper and tomato bacterial streptomyces scabies MLST molecular typing method, and discloses a multilocus sequencing typing (MLST) method for the first time. The method is an operation technology for molecular typing on pepper and tomato bacterial streptomyces scabies. The method comprises the following steps: extracting DNA of genome of pathogenic bacteria, and screening, amplifying, detecting and sequencing seven house-keeping genes; carrying out multi-sequence comparison and analysis on a sequencing result by DNAMAN software to obtain a bacterial strain sequence type; and analyzing the sequence type by using an eBURSTV3 program in an online manner, so that four kinds of pathogenic bacteria can be distinguished effectively. Combination of the seven house-keeping genes and amplification primers which are used in the typing technology are used for the first time, and a PCR reaction system and conditions are optimized voluntarily. The technique process can be used for classification, identification and genetic diversity research of the pepper and tomato bacterial streptomyces scabies.

Description

technical field [0001] The present invention relates to plant pathogenic bacteria molecular typing technique, be specifically related to pepper and tomato bacterial scab (Bacterial Spot caused by Xanthomonads ) method for typing. Background technique [0002] Capsicum and tomato scab is a bacterial disease that commonly occurs on Solanaceae plants. The pathogen mainly damages the leaves, stems, and fruits of peppers and tomatoes, especially on leaves. In the early stage of the disease, small water-soaked, yellow-green spots are formed on the leaves, and after enlargement, they become round or irregular, dark brown, with raised edges and sunken centers, rough and scab-like. In severe cases, the leaves turn yellow, dry, crack, and fall off early. The stems and fruiting stems are diseased, and water-soaked spots are formed at the beginning, and gradually develop into short brown streaks. The lesions are suberized and raised, and the longitudinal fissures are ulcer-like scabs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11G06F19/18
CPCC12Q1/689C12Q2600/156G16B20/00
Inventor 刘清波赵廷昌黄红梅
Owner HUNAN AGRICULTURAL UNIV
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