Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for preparing biologically active peptides by enzymatic hydrolysis of by-products from the processing of blue round trevally

A technology of bioactive peptides and blue round trevally, applied in the field of preparation of bioactive peptides, can solve the problems of marine and terrestrial environmental pollution, less utilization of fish proteolysis, less utilization of processing by-products, etc., and achieves easy hydrolysis process and high realization. The effect of value utilization and low cost

Active Publication Date: 2021-04-02
福州百洋海味食品有限公司
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the growth environment of aquatic organisms is very different from that of land organisms, the amino acid sequence, structure and function of fish proteins are quite different from those of land organisms. Although the current use of proteolysis technology to prepare bioactive peptides The proteins in the world are mainly derived from terrestrial organisms, and the enzymatic hydrolysis of fish proteins is less utilized, but because the amino acid sequence, structure and function of fish proteins are quite different from those of terrestrial organisms, it is possible to obtain more excellent biological activity peptide
Moreover, the ocean occupies 75% of the entire earth, and the protein resources in the ocean far exceed those of land organisms, both in terms of type and quantity, and have not been well developed.
[0003] With the continuous increase of the overall output of aquatic products in China, a large amount of aquatic product processing waste is produced. At present, because enterprises lack effective technical means, the by-products of protein-rich aquatic product processing are rarely used, which not only causes waste of resources, At the same time, it also pollutes the marine and terrestrial environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preparing biologically active peptides by enzymatic hydrolysis of by-products from the processing of blue round trevally
  • A method for preparing biologically active peptides by enzymatic hydrolysis of by-products from the processing of blue round trevally
  • A method for preparing biologically active peptides by enzymatic hydrolysis of by-products from the processing of blue round trevally

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 A kind of method utilizing blue round trevally processing by-product enzymolysis to prepare biologically active peptide

[0028] A method for preparing biologically active peptides by enzymatic hydrolysis of processing by-products of blue round trevally, comprising the following steps:

[0029] (1) Pretreatment: It was determined that the solid content of the by-product of the processing of blue round trevally was 39.4%, the ash content was 1.20%, the total sugar was 2.12%, the fat content was 0.05%, and the crude protein content was 8.17%. Crushing by-products of trevally processing;

[0030] (2) Trypsin hydrolysis: trypsin hydrolysis is added according to the crude protein content, and the specific process conditions are: the ratio of material to water is 1:15, the amount of enzyme added is 7000U / g, the temperature is 50°C, the pH is 7, and the reaction time is 4h;

[0031] (3) Separation of enzymatic hydrolysis solution: centrifuge the enzymatic hydroly...

Embodiment 2

[0033] Embodiment 2 A kind of method utilizing blue round trevally processing by-product enzymolysis to prepare biologically active peptide

[0034] A method for preparing biologically active peptides by enzymatic hydrolysis of processing by-products of blue round trevally, comprising the following steps:

[0035] (1) Pretreatment: It was determined that the solid content of the by-product of the processing of blue round trevally was 39.4%, the ash content was 1.20%, the total sugar was 2.12%, the fat content was 0.05%, and the crude protein content was 8.17%. Crushing by-products of trevally processing;

[0036] (2) Trypsin hydrolysis: trypsin hydrolysis is added according to the crude protein content, and the specific process conditions are: the ratio of material to water is 1:15, the amount of enzyme added is 7450.54U / g, the temperature is 54.48°C, the pH is 7.12, and the reaction time is 4h;

[0037] (3) Separation of enzymatic hydrolysis solution: centrifuge the enzymati...

Embodiment 3

[0039] Embodiment 3 A kind of method that utilizes blue round trevally processing by-product enzymolysis to prepare biologically active peptide

[0040] A method for preparing biologically active peptides by enzymatic hydrolysis of processing by-products of blue round trevally, comprising the following steps:

[0041] (1) Pretreatment: It was determined that the solid content of the by-product of the processing of blue round trevally was 39.4%, the ash content was 1.20%, the total sugar was 2.12%, the fat content was 0.05%, and the crude protein content was 8.17%. Crushing by-products of trevally processing;

[0042] (2) Trypsin hydrolysis: trypsin hydrolysis is added according to the crude protein content, and the specific process conditions are: the ratio of material to water is 1:15, the amount of enzyme added is 8000U / g, the temperature is 60°C, the pH is 8, and the reaction time is 5h;

[0043] (3) Separation of enzymatic hydrolysis solution: centrifuge the enzymatic hyd...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing bioactive peptide by use of byproduct enzymolysis of decapterus maruadsi. The method comprises the following steps: determining protein content in a decapterus maruadsi byproduct, and smashing the decapterus maruadsi byproduct; adding trypsin for enzymolysis according to the coarse protein content; separating the enzymatic hydrolysate by use of an ultrafiltration membrane separator with molecular weight of 3000 Da to obtain the ultra-filtrate, drying the ultra-filtrate to obtain the bioactive peptide. The invention further discloses an application of the prepared bioactive peptide on the oxidation resistance and ACE activity inhibition. The active peptide prepared by use of the biological enzymolysis technology is high in security and free from side effect; not only the high-valued utilization of the leftovers of the aquatic product processing is realized, and the environment pollution can be reduced, thereby producing good economic, ecological and social benefit; the preparation method is simple, easy to operate, low in cost, and convenient for industrial popularization and application.

Description

technical field [0001] The present invention relates to the field of food biotechnology. More specifically, it relates to a method for preparing biologically active peptides by enzymatic hydrolysis of by-products of blue round trevally processing. Background technique [0002] Since the growth environment of aquatic organisms is very different from that of terrestrial organisms, the amino acid sequence, structure and function of fish proteins are quite different from those of terrestrial organisms. Although proteolysis technology is currently used to prepare bioactive peptides The protein is mainly derived from terrestrial organisms, and the enzymatic hydrolysis of fish protein is less utilized. However, because the amino acid sequence, structure and function of fish protein are quite different from those of terrestrial organisms, it is possible to obtain more excellent biological activity. peptides. Moreover, the ocean occupies 75% of the entire earth, and the protein res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P39/06A61P9/12
CPCA61K38/012C07K1/34C12P21/06
Inventor 高向登
Owner 福州百洋海味食品有限公司