A Time-Resolved Fluorescence Immunochromatography Assay
A technology of time-resolved fluorescence and immunochromatography, applied in the direction of analyzing materials, instruments, measuring devices, etc., can solve problems such as inaccurate results, and meet the requirements of reduced driving circuit and processing accuracy, good accuracy, and expected cost requirements Reduced effect
Active Publication Date: 2019-03-05
SHENZHEN ZAITIANYIFANG SCI & TECH LTD
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[0037] In order to solve the problem of inaccurate results caused by displacement errors caused by stepping motors in the prior art, the present invention provides a time-resolved fluorescence immunochromatography method based on displacement measurement optimization
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[0068] The method of the invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0069] Step 1, first in the double-line fluorescent immunoassay reagent strip (see figure 1 ) drop a certain volume of the liquid to be tested into the sample pad, and the chromatography direction is from the test line (T line) to the control line (C line), and wait for the completion of the immunochromatographic reaction.
[0070] Step 2. Next, along the opposite direction of the chromatography, the displacement and fluorescence intensity data are obtained, and a fluorescence intensity-displacement function is established. Wherein, the displacement is acquired through a displacement sensor that moves relative to the double-line fluorescent immunoassay reagent strip.
[0071] In the present invention, the displacement sensor and the "fluorescence excitation-sensing block" are synchronously moved relative to the reagent strip. For examp...
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The invention discloses a time-resolved fluorescence immunochromatography method. The method includes the step that 1, to-be-measured liquid is subjected to chromatography; 2, a fluorescence intensity-displacement function is acquired and established in the chromatography reverse direction; 3, the fluorescence intensity-displacement function obtained in the step 2 is corrected to obtain corrected double-line intensity; 4, according to a fluorescence intensity-concentration relation function of the to-be-measured liquid standard sample, the concentration and a first volume estimated value of the to-be-measured liquid are obtained based on the double-line intensity obtained in the step 3, and a second volume estimated value of a dripped droplet is obtained according to correction; 5, the difference between the two droplet volumes in the step 4 is judged, and corresponding information is output. According to the method, the coordinates of fluorescence are measured through displacement sensor labeling, the fluorescence intensity estimation deviation caused by driving mechanism or transmission mechanism errors is avoided, the influence of movement faults of external movement mechanisms or inaccuracy of mechanical step calculation is avoided, and more accurate target compound concentration information is obtained finally.
Description
technical field [0001] The invention belongs to the field of biological detection, in particular to a time-resolved fluorescence immunochromatography method. Background technique [0002] Time-resolved immunofluorescence is a well-established method for measuring the concentration of target compounds, which utilizes long-lived fluorescent markers to bind the target compounds in the liquid to be tested based on the immunoreaction, and utilizes its long fluorescence extraction time to determine the fluorescence brightness. The measurement, and finally deduce the concentration of the target compound. Immunochromatography is a relatively convenient method of labeling and separating target substances. It uses the principle of chromatography to enrich and separate the markers that are immunocombined with the target compound at different positions on the substrate, and thereby obtain the measurement conditions for the matter. Labels can be colloidal gold, quantum dots, lanthanides...
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IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 曾锐陈淞
Owner SHENZHEN ZAITIANYIFANG SCI & TECH LTD