A method for rapidly obtaining glyphosate-tolerant phenotypes in transgenic maize

A genetically modified corn, glyphosate-tolerant technology, applied in scattering characteristic measurement, color/spectral characteristic measurement, instruments, etc., can solve the problems of unfavorable popularization and application, labor-intensive, material resources, poor timeliness, etc., and achieves simple operation, environmental protection, Ease of operation, avoids the effect of cumbersome processes

Active Publication Date: 2019-06-25
ZHEJIANG UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are destructive to samples, consume a lot of manpower and material resources, and have poor timeliness, which is not conducive to popularization and application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for rapidly obtaining glyphosate-tolerant phenotypes in transgenic maize
  • A method for rapidly obtaining glyphosate-tolerant phenotypes in transgenic maize
  • A method for rapidly obtaining glyphosate-tolerant phenotypes in transgenic maize

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] Further detailed description will be given below in conjunction with the accompanying drawings and embodiments. The specific embodiments are implemented on the premise of the technical solutions of the present invention, and it should be understood that these modes are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The present invention takes maize with transgenic bivalent gene (cry1Ab / cry2Aj-G10evo) as an example, and the identification of processed products of other transgenic crops can refer to the method of this example. Such as figure 1 shown, including the following steps:

[0048] 1. Prepare experimental samples. Plant 120 pots of transgenic (transformed into cry1Ab / cry2Aj-G10evo gene) and non-transgenic corn, and when the corn grows to the 3-leaf stage, the concentration is 1,080g a.e.ha -1 The glyphosate solution was placed in a portable CO2 high-pressure sprayer, and 80 transgenic and non-tran...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for fast obtaining the glyphosate tolerance phenotype of transgenic maizes. The method comprises the steps of preparing experiment samples; obtaining the shikimic acid content of plant canopy leaves of transgenic maizes and non-transgenic maizes suffering glyphosate stress for different days; systematically obtaining hyperspectral data of the transgenic maizes and non-transgenic maizes suffering glyphosate stress for different days, and obtaining the absorbancy of the samples in the visible and near infrared band; performing noise removal processing on the extracted spectroscopic data by a wavelet transform algorithm, and selecting a modeling set and a prediction set through spxy; building an LSSVM (least squares support vector machine) regression analysis model between the maize plant shikimic acid content and the absorbancy by all spectrums; obtaining characteristic spectrums by an SPA method; building the LSSVM regression analysis model between the maize plant shikimic acid content and the absorbancy on the basis of the characteristic spectrums. The method has the advantages that the predication precision is high; the operation is simple; the cost is low and the fast nondestructive detection of the glyphosate tolerance phenotype of the transgenic maize can be realized.

Description

technical field [0001] The invention belongs to the technical field of nondestructive detection of herbicide tolerance of transgenic crops, and in particular relates to a detection method of glyphosate tolerance of transgenic corn using hyperspectral imaging technology. Background technique [0002] With the rapid development of modern agriculture, farmland weeds that affect the development of agricultural production have received widespread attention. Studies have shown that field weeds affect the quality of crops, and can also cause crop yields ranging from 10% to 100% loss, seriously threatening agricultural production. At present, the control of weeds mainly depends on spraying herbicides. Glyphosate is one of the most successful herbicides developed in the 1970s. Because of its wide herbicidal spectrum and excellent systemic conductivity, it can effectively control perennial deep-rooted weeds, is safe for humans and animals, easy to decompose, and does not pollute the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/359G01N21/33G01N21/55G06F17/50
CPCG01N21/33G01N21/359G01N21/55G06F30/20
Inventor 何勇刘小丹冯旭萍刘飞
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap