A method for rapidly obtaining glyphosate-tolerant phenotypes in transgenic maize

Abstract

The invention discloses a method for fast obtaining the glyphosate tolerance phenotype of transgenic maizes. The method comprises the steps of preparing experiment samples; obtaining the shikimic acid content of plant canopy leaves of transgenic maizes and non-transgenic maizes suffering glyphosate stress for different days; systematically obtaining hyperspectral data of the transgenic maizes and non-transgenic maizes suffering glyphosate stress for different days, and obtaining the absorbancy of the samples in the visible and near infrared band; performing noise removal processing on the extracted spectroscopic data by a wavelet transform algorithm, and selecting a modeling set and a prediction set through spxy; building an LSSVM (least squares support vector machine) regression analysis model between the maize plant shikimic acid content and the absorbancy by all spectrums; obtaining characteristic spectrums by an SPA method; building the LSSVM regression analysis model between the maize plant shikimic acid content and the absorbancy on the basis of the characteristic spectrums. The method has the advantages that the predication precision is high; the operation is simple; the cost is low and the fast nondestructive detection of the glyphosate tolerance phenotype of the transgenic maize can be realized.

Description

technical field [0001] The invention belongs to the technical field of nondestructive detection of herbicide tolerance of transgenic crops, and in particular relates to a detection method of glyphosate tolerance of transgenic corn using hyperspectral imaging technology. Background technique [0002] With the rapid development of modern agriculture, farmland weeds that affect the development of agricultural production have received widespread attention. Studies have shown that field weeds affect the quality of crops, and can also cause crop yields ranging from 10% to 100% loss, seriously threatening agricultural production. At present, the control of weeds mainly depends on spraying herbicides. Glyphosate is one of the most successful herbicides developed in the 1970s. Because of its wide herbicidal spectrum and excellent systemic conductivity, it can effectively control perennial deep-rooted weeds, is safe for humans and animals, easy to decompose, and does not pollute the...

AI Technical Summary

Problems solved by technology

However, these methods are destructive to samples, consume a lot of manpower and material resources, and have poor timeliness, which is not conducive to popularization and application.

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