The invention discloses a dust agent with biological pesticides as effective components and a using method thereof. The biological pesticide dust agent adopts raw drugs, mother powders, powder agents, wettable powders, fine granules and other powder preparations of one or more biological pesticides as the effective components, a certain amount of a carrier and auxiliary agents are added, and the biological pesticide dust agent is prepared through air flow pulverizing; the fineness of the biological pesticide dust agent comprises that D90 is less than 53 [mu]m, D50 is less than 37 [mu]m, and D10 is more than 2 [mu]m. The dust agent is applied by a powder spraying method, and can be used for prevention and treatment of diseases and insect pests in facilities or outdoor horticultural plants. After application, the dust agent can perform all-dimensional motion in air and can be lastingly suspended in the air, so that the dust agent can be well diffused, penetrated and go deep to a relatively hidden part in a plant canopy, has high attachment uniformity, and is in favor of playing a better drug effect. In the production application, the using method has the characteristics of water saving, labor saving, effort saving and drug saving, is not affected by climate conditions, and overcomes the disadvantages that a conventional atomizing method adopts much water for drug application and easily induces high wet disease.