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Method for producing blood that divides cells

一种细胞分裂、血液的技术，应用在质谱领域，能够解决破坏全自动分析等问题

Active Publication Date: 2019-03-08
THERMO FINNIGAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biological samples with cellular components can undermine fully automated analysis of the components in the sample, for example by requiring pretreatment to remove cellular components or fragments

Method used

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  • Method for producing blood that divides cells
  • Method for producing blood that divides cells
  • Method for producing blood that divides cells

Examples

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Embodiment Construction

[0032] Embodiments of systems and methods for generating divided blood are described herein.

[0033] The section headings used herein are for organizational purposes only and should not be construed as limiting the described subject matter in any way.

[0034]In this detailed description of various embodiments, for purposes of explanation, numerous specific details are set forth in order to provide a thorough understanding of the disclosed embodiments. It will be apparent, however, to those skilled in the art that these various embodiments may be practiced with or without these specific details. In other instances, structures and devices are shown in block diagram form. Furthermore, those skilled in the art can readily appreciate that the specific order in which methods are presented and performed is illustrative, and it is contemplated that the order may be altered and remain within the spirit and scope of the various embodiments disclosed herein.

[0035] In this applicat...

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Abstract

A method for analyzing a whole blood sample can include injecting whole blood into a first zone of a dual zone sample loop, applying sufficient heat or energy to the whole blood to disintegrate the cellular components of the whole blood sample to produce cell disintegrated blood, and injecting a sufficient volume of the buffer into the dual zone sample loop to move the cell disintegrated blood into a second zone of the dual zone sample loop. The method can further include switching a multiport value to an inject position, flowing the cell disintegrated blood from the dual zone sample loop into a solid phase extraction column, and eluting components of the cell disintegrated blood from the solid phase extraction column into a liquid chromatography column.

Description

technical field [0001] The present invention relates generally to the field of mass spectrometry, including systems and methods for generating divided blood. Background technique [0002] Identifying and quantifying analytes in samples from biological fluids can require complex and lengthy pretreatment procedures to remove cellular components, such as red blood cells, white blood cells, and thrombin cells in whole blood, from the fluid sample. To analyze blood samples, these cellular components may have to be removed by pre-processing procedures such as centrifugation, filtration, sedimentation and / or homogenization by lysis using chemical reagents or mechanical treatment. However, these programs can be difficult to integrate into an automated test format. This also applies where the analyte of interest is present in cellular components, such as immunosuppressive drugs in red blood cells. In this case, the cellular components can be isolated or enriched by centrifugation a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49G01N30/88G01N30/72G01N33/80G01N1/44G01N35/10
CPCG01N33/492G01N1/44G01N30/7233G01N30/88G01N33/491G01N33/80G01N35/1016G01N35/1097G01N2030/8822G01N2035/00475
Inventor J·E·布朗J·J·塔卡如斯基
Owner THERMO FINNIGAN
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