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High-efficiency composite biogas fermentation catalyst

A biogas fermentation and catalyst technology, applied in fermentation, biochemical equipment and methods, waste fuels, etc., can solve the problems of lack of synergistic promotion measures and improvement of the conversion and utilization efficiency of small molecular compounds of methanogens, so as to be conducive to growth and reproduction. , the effect of promoting decomposition rate and improving utilization efficiency

Active Publication Date: 2020-11-24
徐州新南湖科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these schemes are only aimed at the first and second stages of biogas fermentation, that is, to improve the utilization efficiency of fermentation materials. Although this can promote acid production and provide sufficient raw materials for methanogens, it does not improve the ability of methanogens to these The conversion and utilization efficiency of small molecular compounds produced in the acidogenic stage, and there is no overall synergistic promotion measure plan for the three main stages

Method used

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  • High-efficiency composite biogas fermentation catalyst

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] test group:

[0040] Take the purchased Pseudomonas strains and culture them in beef extract peptone medium (add 5.0g peptone, 3.0g beef extract, 5.0gNaCl, 15.0g agar in 1000ml distilled water) for 60-72h at a culture temperature of 28°C. to a concentration of 1x10 9 cfu / ml;

[0041] Take the purchased Bacillus subtilis subsp. subsp. and culture it in beef extract peptone medium (add 5.0g peptone, 3.0g beef extract, 5.0g NaCl, 15.0g agar in 1000ml distilled water) for 60-72h at a culture temperature of 28°C. to a concentration of 1x10 9 cfu / ml;

[0042] Take the purchased Acetobacter pasteurii, add 100g glucose, 10g yeast extract, 20g CaCO to YPD medium (1000ml distilled water 3 , 15g agar, adjust the pH to 6.8) for 80 hours, culture temperature 28 ℃, to a concentration of 1x10 9 cfu / ml;

[0043] Take purchased Saccharomyces cerevisiae and culture it in 5Bé wort agar medium (1.0L 5Bé wort with 15.0g agar added, natural pH) for 75h at a temperature of 28-30°C to a ...

Embodiment 2

[0057] test group:

[0058] Take the purchased Pseudomonas strains and culture them in beef extract peptone medium (add 5.0g peptone, 3.0g beef extract, 5.0gNaCl, 15.0g agar in 1000ml distilled water) for 60-72h at a culture temperature of 28°C. to a concentration of 1x10 9 cfu / ml;

[0059] Take the purchased Bacillus subtilis subsp. subsp. and culture it in beef extract peptone medium (add 5.0g peptone, 3.0g beef extract, 5.0g NaCl, 15.0g agar in 1000ml distilled water) for 60-72h at a culture temperature of 28°C. to a concentration of 1x10 9 cfu / ml;

[0060] Take the purchased Acetobacter pasteurii, add 100g glucose, 10g yeast extract, 20g CaCO to YPD medium (1000ml distilled water 3 , 15g agar, adjust the pH to 6.8) for 80 hours, culture temperature 28 degrees, to a concentration of 1x10 9 cfu / ml;

[0061] Take purchased Saccharomyces cerevisiae and culture it in 5Bé wort agar medium (1.0L 5Bé wort with 15.0g agar added, natural pH) for 75h at a temperature of 28-30°C...

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Abstract

The invention relates to a high-efficiency compound biogas fermentation catalyst which is prepared from a complex microbial inoculant, an enzyme activator and reducing salt, wherein the enzyme activator and the reducing salt are added into the complex microbial inoculant. The complex microbial inoculant in the fermentation catalyst disclosed by the invention can generate enzymes in a biogas fermentation corresponding stage, the enzymes include cellulase and amylase, the enzymes can improve enzyme activity through the enzyme activator, and decomposing utilization efficiency of all the raw materials in a biogas digester is effectively improved; saccharomycete and acetic bacteria in the complex microbial inoculant can improve accumulation of important intermediate metabolite of acetic acid; the reducing salt in the fermentation catalyst can reduce an oxidation reduction potential of a biogas production system and reduce dissolved oxygen, a strict anaerobic environment condition is provided for methanogens, high-efficiency methane production of the methanogens is facilitated, and biogas yield of the biogas digester is effectively improved.

Description

technical field [0001] The invention relates to the field of biogas fermentation, in particular to a high-efficiency composite biogas fermentation catalyst. Background technique [0002] Biogas fermentation is a process in which a variety of organic matter is used as raw materials and biogas is finally generated through a series of complex fermentations of microorganisms. [0003] The biogas anaerobic fermentation process is a complex biochemical reaction process in which a variety of microorganisms combine and interact alternately. Methanogens and non-methanogens restrict and depend on each other. [0004] The biogas fermentation process has the following three stages: [0005] The first stage [0006] In the liquefaction stage, microorganisms (cellulolytic bacteria, proteolytic bacteria, etc.) convert solid organic matter into soluble organic matter through the action of extracellular enzymes. [0007] second stage [0008] In the acid production stage, soluble substan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C12P5/02C12R1/38C12R1/125C12R1/02C12R1/865
CPCC12N1/18C12N1/20C12P5/023Y02E50/30
Inventor 王万能成福徐良
Owner 徐州新南湖科技有限公司
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